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Iranian Journal of Pathology، جلد ۱۲، شماره ۱، صفحات ۵۳-۶۱
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Investigation of Efflux-Mediated Tetracycline Resistance in Shigella Isolates Using the Inhibitor and Real Time Polymerase Chain Reaction Method
چکیده انگلیسی مقاله Background: Shigella spp. are gram negative bacteria, which are of global public health importance. The growing of multidrug-resistant Shigella isolates are a major problemaround the world. Methods: Overall, 50 isolates of Shigella spp. from children diarrheic stools were studied. The isolates were identified and confirmed using biochemical, serological and molecular methods (ipaH, wbgZ and rfc genes). Antimicrobial susceptibility test was done according to the Clinical and Laboratory Standards Institute (CLSI) guidelines against minocycline, tetracycline, doxycycline, ampicillin, streptomycin, trimethoprim-sulfamethoxazole, nalidixic acid, norfloxacin, ciprofloxacin and levofloxacin. Also, the role of efflux pump in defense of Shigella against tetracycline was investigated by Minimum Inhibitory Concentration (MIC) with and without an efflux pump inhibitor. Detection of tetA, tetB, tetC and tetD genes in Shigella was evaluated by conventional Polymerase Chain Reaction (PCR) and real time PCR. Results: Molecular identification revealed a prevalence of 14% for Shigella flexneri and 86% for Shigella sonnei. Minimum Inhibitory Concentration (MIC) of 90% of resistant isolates was changed in the presence CCCP.Results of conventional PCR exhibited that 66% of isolates were positive for tetA, while according to real time PCR method, 90% of isolates carried tetA. Positive results for tetB were12% and 18% by conventional and real time PCR methods, respectively. No positive results were detected for tetC and tetD. Also, tetB was detected only in S. flexneri while tetA was detected in both S. flexneri and S. sonnei. Conclusion: It seems that efflux-mediated tetracycline resistance to tetracycline in S. flexneri can be related to tetB, however resistance in S. sonnei can be related to the expression of tetA.
کلیدواژه‌های انگلیسی مقاله Shigella,Tetracycline resistance,Efflux pump,Real-Time PCR
نویسندگان مقاله شادی شاهسون |
antimicrobial resistance research center, iran university of medical science, tehran, iran

پرویز اولیا |
molecular microbiology research center, shahed university, tehran, ir iran
سازمان اصلی تایید شده: دانشگاه شاهد (Shahed university)

عبدالعزیز رستگار لاری | rastegar lari
dept. of microbiology, faculty of medicine, iran university of medical science, tehran, iran
سازمان اصلی تایید شده: دانشگاه شاهد (Shahed university)

بیتا بخشی |
dept. of microbiology, faculty of medicine, tarbiat modares university, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)

ملیحه نوبخت |
antimicrobial resistance research center, iran university of medical science, tehran, iran
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

نشانی اینترنتی http://ijp.iranpath.org/article_24218_ace747ab61f9851a9e3b65312bf0ea2c.pdf
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/194/article-194-323928.pdf
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده 1
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