| چکیده انگلیسی مقاله |
Objective(s): Some species of Prunus L. are popularly used to treat gastric ulcers. However, the possible healing mechanisms of the anti-ulcer activity of P. spinosa, which has proven antioxidant, anti-inflammatory, and wound-healing properties, are unclear.Materials and Methods: Ethanol extracts of P. spinosa fruits were administered orally at 100 mg/kg and 200 mg/kg to Wistar albino rats, with an indomethacin-induced gastric ulcer model. The ulcerous areas on the stomach surface were examined macroscopically. Tissues were examined histopathologically and biochemically. LC-HRMS revealed the phytochemical content.Results: TNF-α, IL-6, IL-1β, IL-8, and NF-kB levels were higher in the gastric ulcer group than in the extract groups. The VEGF values did not differ in each group. A significant difference was found between the lansoprazole group and the high-dose P. spinosa group regarding PGE2 levels. A histopathologically significant difference was observed between the healthy group and the indomethacin-applied groups in terms of neutrophilic infiltration of the gastric mucosa. Ascorbic acid (1547.521 µg/g), homoprotocatechuic acid (1268.217 µg/g), and genistein (1014.462 µg/g) were found as the main compounds in the P. spinosa extract by LC-HRMS.Conclusion: Our results demonstrated that P. spinosa protected the gastric mucosa from inflammation and also modulated the PGE2 pathway. When considered in terms of TNF-α, IL-1β, IL-8, IL-6, PGE2, and NF-kB values, it can be concluded that it has a similar or even more positive effect than the reference substance. P. spinosa showed its effects in a dose-dependent manner. |
| نویسندگان مقاله |
| Nihal Cetin
Department of Pharmacology, Faculty of Medicine, Selcuk University, 42131, Konya, Turkey
| Esma Menevse
Department of Biochemistry, Faculty of Medicine, Selcuk University, 42131, Konya, Turkey
| Cengizhan Ceylan
Department of Clinical Pharmacy, Faculty of Pharmacy, Selcuk University, 42100, Konya, Turkey
| Zeliha Esin Celik
Department of Pathology, Faculty of Medicine, Selcuk University, 42131, Konya, Turkey
| Neriman Akdam
Department of Biostatistics, Faculty of Medicine, Selcuk University, 42131, Konya, Turkey
| Seyma Tetik Rama
Research Assistant, Department of Pharmacology, Faculty of Pharmacy, Selcuk University, 42100, Konya, Turkey
| Tugsen Buyukyıldırım
Research Assistant, Department of Pharmacognosy, Faculty of Pharmacy, Selcuk University, 42100, Konya, Turkey
| Leyla Paşayeva
Department of Pharmacognosy, Faculty of Pharmacy, Erciyes University, 38280, Kayseri, Turkey
| Osman Tugay
Department of Pharmaceutical Botany, Faculty of Pharmacy, Selcuk University, 42100, Konya, Turkey
| Meltem Gumus
Department of Pediatrics, Division of Pediatric Gastroenterology, Faculty of Medicine, Selcuk University, 42131, Konya, Turkey
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