این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Medical Physics، جلد ۱۴، شماره ۱، صفحات ۲۹-۳۷
عنوان فارسی
چکیده فارسی مقاله
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عنوان انگلیسی Molecular Effects of Atmospheric Pressure Plasma Jet on the Double-Stranded DNA
چکیده انگلیسی مقاله Introduction The aim of this study was toinvestigate the sterilization potential of atmospheric pressure plasma jet (APPJ) and interactions of this technology with double-stranded DNA using the polymerase chain reaction (PCR) and single-strand conformation polymorphism (SSCP) techniques. Materials and Methods The plasma jet was produced through a high voltage sinusoidal power supplyusing a mixture of argon and oxygen gases with theflow rate of 1 L/min. Escherichia coli cells and double-stranded DNA (dsDNA) fragments were amplified by T7 universal primer through the PCR technique and treated with argon/oxygen APPJ at different exposure times. The data were analyzed by the agarose and polyacrylamide gel electrophoresis, SSCP and renewed PCR techniques. Results According to the results of the study, the APPJ could serve as an effective instrument for sterilization at > 30 sec discharge. The destruction of DNA was detectable by different techniques after 120 sec from APPJ discharge. Conclusion Our findings revealed that the active species of plasma can lead to cell death. These species may break or nick the dsDNA, exchange DNA nucleotides, and lead to transition and transversion mutations. These mutagenesis effects of APPJ might be the reason of microorganism cell death after the treatment in addition to other destructive effects of APPJ on macromolecules. 
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نویسندگان مقاله اباصلت حسین زاده کلاگر | abasalt hosseinzadeh colagar
department of molecular and cell biology, faculty of basic sciences, university of mazandaran

سازمان اصلی تایید شده: دانشگاه مازندران (Mazandaran university)

سید محسن مرتضوی | seyed mohsen mortazavi
department of molecular and cell biology, faculty of basic sciences, university of mazandaran

سازمان اصلی تایید شده: دانشگاه مازندران (Mazandaran university)

وحید عرب یارمحمدی | vahid arab yarmohammadi
department of molecular and cell biology, faculty of basic sciences, university of mazandaran

سازمان اصلی تایید شده: دانشگاه مازندران (Mazandaran university)

فرشاد صحبت زاده | farshad sohbatzadeh
department of atomic and molecular physics, faculty of basic sciences, university of mazandaran

سازمان اصلی تایید شده: دانشگاه مازندران (Mazandaran university)

نشانی اینترنتی http://ijmp.mums.ac.ir/article_8461.html
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/145/article-145-326806.pdf
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Paper
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