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Physiology and Pharmacology، جلد ۲۰، شماره ۴، صفحات ۲۳۹-۲۴۵
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی High glucose condition down-regulates the inhibitory G-protein subunit, Gαi, in pheochromocytoma PC12 cells
چکیده انگلیسی مقاله Introduction: G-proteins have an important role in the cell signaling of numerous receptors. The situation of G-proteins in health and disease and their critical role in the development of diabetic side effects is an interested scientific field. Here, the changes in the expression of G-protein subunits (Gαi, Gαs and Gβ) were evaluated in hyperglycemic situation of PC12 cells as a cellular model for the induction of diabetic side effect. Methods: Rat pheochromocytoma PC12 cells were grown in normal or high-glucose (4X normal glucose) medium. Cell viability was determined by MTT assay and the generation of intracellular reactive oxygen species (ROS) studied using fluorescence spectrophotometry. RT-PCR and immunobloting were performed to evaluate the expression of specific G-protein subunits in the levels of mRNA and protein, respectively. Results: In high glucose condition (100 mM glucose for 48h), the cell viability was significantly decreased and intracellular ROS increased. In addition, Gαi expression level was significantly decreased in hyperglycemic PC12 cells. However, the levels of Gαs and Gβ mRNAs and their proteins were not altered in high glucose-treated cells. Conclusion: The results demonstrate that deregulation or disruption in the signaling of Gai coupled receptors can be occurred in hyperglycemic condition.
کلیدواژه‌های انگلیسی مقاله G-proteins, Gene expression, Hyperglycemia, PC12 cells
نویسندگان مقاله سعید اسماعیلی ماهانی | saeed esmaeili mahani
department of biology, faculty of sciences, shahid bahonar university of kerman, kerman, iran

سازمان اصلی تایید شده: دانشگاه شهید باهنر (Shahid bahonar university)

زهرا حاجی علیزاده | zahra hajializadeh
laboratory of molecular neuroscience, kerman neuroscience research center, kerman university of medical sciences, kerman, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمان (Kerman university of medical sciences)

شیما ترک زاده ماهانی | shima torkzadeh mahani
laboratory of molecular neuroscience, kerman neuroscience research center, kerman university of medical sciences, kerman, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمان (Kerman university of medical sciences)

نشانی اینترنتی http://phypha.ir/ppj/browse.php?a_code=A-10-79-9&slc_lang=fa&sid=en
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/26/article-26-327734.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Cellular and Molecular BioMedicine
نوع مقاله منتشر شده Original Research
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