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Veterinary Research Forum، جلد ۱۵، شماره ۱۰، صفحات ۵۵۹-۵۶۴
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عنوان انگلیسی Investigating hydropericardium syndrome with different histopathological techniques in broiler chickens
چکیده انگلیسی مقاله Hydropericardium syndrome (HPS) has caused significant financial losses to the Iranian poultry industry in the past few years. Thirty-two broiler chickens with gross lesions of HPS were inspected histologically and immunohistochemically. Sampling was performed in Sabzevar, Iran. The dead and sick birds from random farms were subjected to necropsy examinations. Only four broiler chickens had no hydropericardium and the other gross findings were similar for birds. Basophilic and eosinophilic intranuclear inclusions, hemorrhages and necrosis in different organs were the primary characteristic histologic lesions. Lymphoid depletion, goblet cell hyperplasia and necrotizing enteritis were some of the findings reported in previous research. Low macrophage infiltration rate and brain lesions were other discoveries in Hematoxylin and Eosin (H&E) examination. Feulgen reaction and Cluster of Differentiation 68 (CD68) immunohistochemical staining were used for a comprehensive investigation and these techniques revealed improved histopathologic details. Feulgen staining confirmed brain lesions and some other changes in different organs. Eventually, the CD68 method revealed low macrophage presence in most organs. This study suggested that HPS might cause brain damage and the susceptibility of the Arian breed to the adenovirus needs further investigation.
کلیدواژه‌های انگلیسی مقاله Feulgen staining,Histopathology,Hydropericardium syndrome,Immunohistochemistry,Iran
نویسندگان مقاله Hossein Alavizadeh |
Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

Zahra Moosavi |
Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

Gholamreza Hashemi Tabar |
Department of Biotechnology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

نشانی اینترنتی https://vrf.iranjournals.ir/article_715830_a1c7d2a4181907599cbe6ca703459761.pdf
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