Background and Objectives: The aim of this study was to investigate the effect of sheep lymph nodes and antibiotics on the bacterium that causes acne and the bacteria that usually exist with it.  Materials and Methods: In this laboratory study, 24 patients with acne (18 to 45 years old) were selected to isolate the bacteria. Also, 24 clinical isolates of Staphylococcus aureus and Staphylococcus epidermidis were isolated from the wound samples of patients. After culturing the bacteria, their sensitivity to common antibiotics was evaluated. Data were reported as percentage.  Results: The results showed that Cutibacterium acnes and Staphylococcus aureus were 100% sensitive to vancomycin. Also, Cutibacterium acnes showed sensitivity in tetracycline and imipenem groups in 95% of cases and in cotrimoxazole and piperacillin/tazobactam groups in 90% of cases. In addition, the highest sensitivity of Staphylococcus epidermis to piperacillin/tazobactam (62.5%) and the highest drug resistance (91.67%) were related to azithromycin and erythromycin groups.  Conclusion: Due to the increase in drug resistance, it is suggested to use antibiotics with the least drug resistance such as vancomycin and imipenem in the treatment of acne.  Keywords: Lymph node, Antibiotic, Acne, Staphylococcus aureus, Cutibacterium acnes    Funding: This study was funded by Rafsanjan University of Medical Sciences.  Conflict of interest: None declared.  Ethical considerations: The Ethics Committee of Rafsanjan University of Medical Sciences approved the study (IR.RUMS.REC.1399.103).    Authors’ contributions:  - Conceptualization: Mohammad Mohsen Taghavi, Ebrahim Rezazadeh Zarandi  - Methodology: Mohammad Mohsen Taghavi, Ahmad Shabanizadeh  - Data collection: Mahnaz Ramezani, Amir Hesam Khodaei  - Formal analysis: Abdolreza Babaee, Samereh Dehghani-Soltani  - Supervision: Mohammad Mohsen Taghavi, Zahra Taghipour  - Project administration: Mohammad Mohsen Taghavi  - Writing - original draft: Abdolreza Babaee, Ebrahim Rezazadeh Zarandi  - Writing - review & editing: Mohammad Mohsen Taghavi, Abdolreza Babaee   
Citation: Babaee A, Rezazadeh Zarandi E, Shabanizadeh E, Taghipour Z, Dehghani-Soltani S, Ramezani M, Khodaei AH, Taghavi MM. Investigation of Inhibitory Effect of Sheep Lymph Nodes and Antibiotics on Cutibacterium Acnes, Staphylococcus Aureus, and Staphylococcus Epidermidis in the Laboratory Condition: A Short Report. J Rafsanjan Univ Med Sci 2024; 23 (6): 568-75. [Farsi]