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Iranian Journal of Basic Medical Sciences، جلد ۲۷، شماره ۱۲، صفحات ۱۵۳۶-۱۵۴۶
عنوان فارسی
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عنوان انگلیسی Regenerative role of mast cells and mesenchymal stem cells in histopathology of the sciatic nerve and tibialis cranialis muscle, following denervation in rats
چکیده انگلیسی مقاله Objective(s): Atrophy of the muscles following denervation can lead to the death of myofibers. This study evaluated the sciatic nerve and tibialis cranialis muscle (TCM) regeneration using scaffold and cells. Materials and Methods: Ninety adult male Wistar rats were divided into six main groups (n=15) and three subgroups (2, 4, and 8 weeks). Groups: control; without surgery, Tr; sciatic nerve transected in silicone tube, S; collagen gel put inside the silicone tube, MC; placed 3×104 mast cells mixed with scaffold, MSC; placed 3×104 mesenchymal stem cells mixed with scaffold, and MC+MSC; 3×104 of each of the mast cell and mesenchymal stem cells along with scaffold. Animals were euthanized and sampled for muscle and nerve histological and nerve immunohistochemical evaluations. Results: Diameter of muscle fibers, ratio of the muscle fiber’s nuclei to the fibrocyte nuclei (mn/fn), ratio of the muscle fibers nuclei number to the muscle fiber’s number (mn/mf), and ratio of the blood vessels number to the number of muscle fibers (v/mf) in all treatment groups, especially the MC + MSC group, increased compared to the Tr group but the number of mast cells, the percentage of sarcoplasmolysis, and necrosis fibers decreased. Histomorphometric results of the nerve in its various parts and immunohistochemistry results also showed improved nerve conduction, especially in the MC + MSC group. Conclusion: In this study, nerve improvement happened mainly for two reasons: cells and time. So, the most obvious improvement occurred in the group with mast and mesenchymal cells in the 8th week.
کلیدواژه‌های انگلیسی مقاله Denervation, Histology, Mast cells, Mesenchymal stem cells, Muscle, Rat, Regeneration
نویسندگان مقاله | Zahra Bakhtiary
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran|Department of Histology, Faculty of Veterinary Sciences, Ilam University, Ilam, Iran


| Rasoul Shahrooz
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran


| Rahim Hobbenaghi
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran


| Saeed Azizi
Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran


| Farhad Soltanalinejad
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran


| Ali Baradar Khoshfetrat
Department of Chemical Engineering, Sahand University of Technology, Tabriz, Iran
نشانی اینترنتی https://ijbms.mums.ac.ir/article_24737.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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