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Iranian Journal of Biotechnology، جلد ۲۲، شماره ۳، صفحات ۱-۱۳
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عنوان انگلیسی Hypoxic Preconditioning Prevents Oxidative Stress-Induced Cell Death in Human Hair Follicle Stem Cells
چکیده انگلیسی مقاله Background: Cell therapy, involving the transplantation of viable cells for therapeutic purposes, offers immense promise but faces challenges related to cell survival and functionality post-transplantation. Preconditioning strategies, particularly hypoxic preconditioning, have emerged as a means to enhance cell adaptability and resilience.

Objectives: This study investigated the impact of hypoxic preconditioning on the survival and oxidative stress tolerance of nestin-expressing hair follicle stem cells (hHFSCs) and SH-SY5Y neuroblastoma cells, two crucial cell types for central nervous system therapies. The study also examined the relative expression of three key genes, HIF1α, BDNF, and VEGF following hypoxic preconditioning.

Materials and Methods: hHFSCs were isolated from human hair follicles, characterized, and subjected to hypoxia for up to 72 hours. SH-SY5Y cells were similarly preconditioned for up to 72 hours. Cell viability under hypoxic conditions and oxidative stress was assessed. The relative expression of key genes was evaluated using qRT-PCR.

Results: hHFSCs exhibited remarkable resilience to hypoxic conditions, while SH-SY5Y cells displayed lower tolerance. Hypoxic preconditioning improved the viability of both cell types under oxidative stress. HIF1α mRNA was significantly downregulated, and VEGF transcripts increased after preconditioning, suggesting adaptations to prolonged hypoxia.

Conclusion: Hypoxic preconditioning enhances the survival and oxidative stress resilience of hHFSCs and SH-SY5Y cells, offering potential benefits for central nervous system cell therapy. The differential responses observed emphasize the need for tailored preconditioning strategies for specific cell types. These findings underscore the importance of hypoxic preconditioning and warrant further research into the underlying mechanisms, bringing us closer to effective neurological disorder treatments.
کلیدواژه‌های انگلیسی مقاله EPI-NCSCs,HAP stem cells,hypoxia,priming
نویسندگان مقاله Mohammad Saied Salehi |
Clinical Neurology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

Fatemeh Changiz Khani |
Department of Biology, Zarghan Branch, Islamic Azad University, Zarghan, Iran

Sanaz Ansari |
Department of Biology, Zarghan Branch, Islamic Azad University, Zarghan, Iran

Mohammad Javad Mokhtari |
Zarghan Branch, Islamic Azad University, Zarghan, Iran

Mahintaj Dara |
Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

Mahnaz Bayat |
Clinical Neurology Research Center, Shiraz University of Medical Sciences, Shiraz Iran

Etrat Hooshmandi |
Clinical Neurology Research Center, Shiraz University of Medical Sciences, Shiraz Iran

Nahid Ashjazadeh |
Clinical Neurology Research Center, Shiraz University of Medical Sciences, Shiraz Iran

Afshin Borhani-haghighi |
Clinical Neurology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

Gökhan Ünal |
Department of Pharmacology, Faculty of Pharmacy, Erciyes University, Kayseri, Turkey

Sareh Pandamooz |
Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

نشانی اینترنتی https://www.ijbiotech.com/article_205587_a498a06905eeb7f324b058e12c4333cc.pdf
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