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Iranian Journal of Biotechnology، جلد ۲۲، شماره ۳، صفحات ۱۴-۲۲
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عنوان انگلیسی LncRNA U731166 increases the accumulation of TGFBR1 by sponging miR-3607-3p in esophageal squamous-cell carcinomas (ESCC) to promote tumor metastasis
چکیده انگلیسی مقاله Background: Long non-coding RNA (lncRNA) U731166 and microRNA (miR)-3607-3p are two ncRNAs with critical roles in cancer biology, while their involvement in esophageal squamous-cell carcinomas (ESCC) is unclear. We predicted that U731166 and miR-3607-3p might interact with each other. This study was carried out to explore their role and interaction in ESCC.

Objectives: The present study was therefore conducted to explore the involvement of U731166 and miR-3607-3p in ESCC, with a focus on the interaction between them.

Materials and Methods: Paired ESCC and non-tumor tissue samples were collected from 72 ESCC patients. Expression of U731166 and miR-3607-3p in paired tissues was measured by RT-qPCR. The direct interaction between U731166 and miR-3607-3p was detected by performing RNA-RNA pulldown assay. Overexpression was performed to explore the role of U731166 and miR-3607-3p in regulating the expression of each other. The role of U731166 and miR-3607-3p in regulating the expression of TGFBR1 was assessed by RT-qPCR and Western blot analysis. Cell invasion and migration were analyzed by Transwell assays.

Results: Compared to non-tumor tissues, U731166 was highly upregulated in ESCC, while miR-3607-3p was downregulated in ESCC. U731166 and miR-3607-3p directly interacted with each other, but they are not closely correlated and did not regulate the expression of each other. Moreover, U731166 suppressed the role of miR-3607-3p in downregulating TGFBR1 and inhibiting cancer cell invasion and migration. U731166 and miR-3607-3p were closely associated with patients’ tumor metastasis but not tumor size.

Conclusion: U731166 may upregulate TGFBR1 by sponging miR-3607-3p in ESCC cells to promote tumor metastasis.
کلیدواژه‌های انگلیسی مقاله esophageal squamous-cell carcinomas,U731166,TGFBR1,miR-3607-3p
نویسندگان مقاله Mingbo Wang |
Department of Thoracic Surgery， The Fourth Hospital of Hebei Medical University Shijiazhuang City, Hebei Province, 050000, PR. China

Meng Wang |
Thoracic surgery Department，Tianjin Chest hospital, Tianjin City, 300222, PR. China

Chao Huang |
Department of Thoracic Surgery， The Fourth Hospital of Hebei Medical University Shijiazhuang City, Hebei Province, 050000, PR. China

Yonggang Zhu |
Department of Thoracic Surgery， The Fourth Hospital of Hebei Medical University Shijiazhuang City, Hebei Province, 050000, PR. China

Fan Zhang |
Department of Thoracic Surgery， The Fourth Hospital of Hebei Medical University Shijiazhuang City, Hebei Province, 050000, PR. China

Wenda Gao |
Department of Thoracic Surgery， The Fourth Hospital of Hebei Medical University Shijiazhuang City, Hebei Province, 050000, PR. China

Zhenhua Li |
Department of Thoracic Surgery， The Fourth Hospital of Hebei Medical University Shijiazhuang City, Hebei Province, 050000, PR. China

Liangbiao Peng |
Department of Thoracic Surgery, the fourth hospital of handan, Hebei Province，056200, PR. China

Ziqiang Tian |
Department of Thoracic Surgery， The Fourth Hospital of Hebei Medical University Shijiazhuang City, Hebei Province, 050000, PR. China

Chao Gao |
Department of Radiation Oncology，The Fourth Hospital of Hebei Medical University, Shijiazhuang City, Hebei Province, 050011, PR. China

Xingpeng Han |
Thoracic surgery Department，Tianjin Chest hospital, Tianjin City, 300222, PR. China

نشانی اینترنتی https://www.ijbiotech.com/article_205575_fc87c0a92d041d3442c1e395b5397eae.pdf
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