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Iranian Journal of Biotechnology، جلد ۲۲، شماره ۳، صفحات ۶۲-۷۷
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عنوان انگلیسی Inhibition of chitinase-3-like protein 1 reduced epithelial–mesenchymal transition and vascular epithelial cadherin expression in oesophageal squamous cell carcinoma
چکیده انگلیسی مقاله Background: Oesophageal cancer (OC) is one of the common malignant tumors, and the prognosis of patients is poor. Further exploration of OC pathogenesis remains warranted.

Objective: The relationship between vascular epithelial cadherin (VE-cadherin) and chitinase-3-like protein 1 (CHI3L1) in OC is currently unknown. To further explore the relationship, immunohistochemical staining was performed to detect the expression level of CHI3L1 and VE-cadherin in oesophageal squamous cell carcinoma (OSCC).

Methods: Small interfering RNAs (siRNAs) inhibited CHI3L1 expression in KYSE-150 and TE1 cells. Western blot and quantitative fluorescence polymerase chain reaction were used to detect the levels of CHI3L1, VE-cadherin and epithelial–mesenchymal transition (EMT)-related proteins in vitro and in vivo, and KYSE-150 cells were used to establish an in-vivo model and observe tumour growth.

Results: High levels of CHI3L1 and VE-cadherin expression were closely associated with the progression of OSCC; the pathologic tumour-node-metastasis stage was also closely related with the progression of OSCC (p < 0.05). High levels of CHI3L1 and VE-cadherin expression led to poor prognosis in patients with OC. In KYSE-150 and TE1 OC cell lines, the invasion, migration and proliferation of cells decreased, and the apoptotic rate increased after CHI3L1 expression was decreased using siRNA. The CHI3L1, VE-cadherin, Snail, Twist1 protein and mRNA expression levels decreased, whereas the E-cadherin levels increased.

Conclusions: Chitinase-3-like protein 1 could promote the EMT of OSCC, and the inhibition of CHI3L1 decreases the expression of VE-cadherin, which inhibits tumour angiogenesis and tumour progression in OSCC.
کلیدواژه‌های انگلیسی مقاله CHI3L1,VE-cadherin,oesophageal cancer,Squamous Cell Carcinoma
نویسندگان مقاله Yanzi Qin |
Department of Pathology, The First Affiliated Hospital of Bengbu Medical College

Wenjun Zhao |
Department of Emergency Internal Medicine, The Third the People′s Hospital of Bengbu

Qicai Li |
Department of Thoracic and Cardiovascular Surgery, The First Affiliated Hospital of Bengbu Medical College

Zhaogeng Cai |
Department of Pathology, The First Affiliated Hospital of Bengbu Medical College

Guowen Wang |
Department of Thoracic and Cardiovascular Surgery, The First Affiliated Hospital of Bengbu Medical College

Nan Wang |
Department of Critical Care Medicine, The Fourth Affiliated Hospital of Anhui Medical University

Minjae Jung |
Department of Biotechnology, Sangmyung University, 20 Hongjimun 2-gil, Jongno-gu, Seoul 03016, Korea

نشانی اینترنتی https://www.ijbiotech.com/article_205578_e2736d3f39e53e866c5d2ea1bb53410b.pdf
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