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Iranian Biomedical Journal، جلد ۲۸، شماره ۴، صفحات ۱۵۶-۱۶۷
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چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی In silico and in vivo Investigations of the Immunoreactivity of Klebsiella pneumoniae OmpA Protein as a Vaccine Candidate
چکیده انگلیسی مقاله
Background: The growing threat of antibiotic resistance and Klebsiella pneumoniae infection in healthcare settings highlights the urgent need for innovative solutions, such as vaccines, to address these challenges. This study sought to assess the potential of using K. pneumoniae outer membrane protein A (OmpA) as a vaccine candidate through both in silico and in vivo analyses.
Methods: The study examined the OmpA protein sequence for subcellular localization, antigenicity, allergenicity, similarity to the human proteome, physicochemical properties, B-cell epitopes, MHC binding sites, tertiary structure predictions, molecular docking, and immune response simulations. The ompA gene was cloned into the pET-28a (+) vector, expressed, purified and confirmed using Western blotting analysis. IgG levels in the serum of the immunized mice were measured using ELISA with dilutions ranging from 1:100 to 1:6400, targeting recombinant outer membrane protein A (rOmpA) and K. pneumoniae ATCC 13883. The sensitivity and specificity of the ELISA method were also assessed.
Results: The bioinformatics analysis identified rOmpA as a promising vaccine candidate. The immunized group demonstrated significant production of specific total IgG antibodies against rOmpA and K. pneumoniae ATCC1 13883, as compared to the control group (p < 0.0001). The titers of antibodies produced in response to bacterial exposure did not show any significant difference when compared to the anti-rOmpA antibodies (p > 0.05). The ELISA test sensitivity was 1:3200, and the antibodies in the serum could accurately recognize K. pneumoniae cells.
Conclusion: This study is a significant advancement in the development of a potential vaccine against K. pneumoniae that relies on OmpA. Nevertheless, additional experimental analyses are required.
کلیدواژه‌های انگلیسی مقاله Klebsiella pneumoniae, Outer membrane protein, Vaccine
نویسندگان مقاله | shahla shahbazi
Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran


| Farzad Badmasti
Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran


| Mehri Habibi
Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran


| Samira Sabzi
Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran


| Narjes Noori Goodarzi
Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran


| Mehdi Farokhi
National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran


| Mohammad Reza Asadi Karam*
Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran
نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-5562-1&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Molecular Microbiology
نوع مقاله منتشر شده مقاله کامل
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