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Iranian Biomedical Journal، جلد ۲۷، شماره ۵، صفحات ۳۲۰-۳۲۵
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عنوان انگلیسی Optimal Extraction and Deproteinization Method for Mannoprotein Purification from Kluyveromyces marxianus
چکیده انگلیسی مقاله Background: Mannoproteins, mannose-glycosylated proteins, play an important role in biological processes and have various applications in industries. Several methods have been already used for the extraction of mannoproteins from yeast cell-wall. The aim of this study was to evaluate the extraction and deproteinization of mannan oligosaccharide from the Kluyveromyces (K.) marxianus mannoprotein.
Methods: To acquire crude mannan oligosaccharides, K. marxianus mannoproteins were deproteinized by the Sevage, trichloroacetic acid, and hydrochloric acid (HCL) methods. Total nitrogen, crude protein content, fat, carbohydrate and ash content were measured according to the monograph prepared by the meeting of the Joint FAO/WHO Expert Committee and standard. Mannan oligosaccharide loss, percentage of deproteinization, and chemical composition of the product were assessed to check the proficiency of different methods.
Results: Highly purified (95.4%) mannan oligosaccharide with the highest deproteinization (97.33 ± 0.4%) and mannan oligosaccharide loss (25.1 ± 0.6%) were obtained following HCl method.
Conclusion: HCl, was the most appropriate deproteinization method for the removal of impurities. This preliminary data will support future studies to design scale-up procedures.
کلیدواژه‌های انگلیسی مقاله Kluyveromyces marxianus, Mannan oligosaccharides, Downstream extraction process
نویسندگان مقاله | Ashraf Hajhosseini
Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran


| Anoosheh Sharifan
Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran


| Zohreh Eftekhari
Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran


| Ariana Alavi
Production and Research Complex, Pasteur Institute of Iran, Tehran, Iran


| Delaram Doroud
Production and Research Complex, Pasteur Institute of Iran, Tehran, Iran
نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-2110-1&slc_lang=en&sid=1
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