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Iranian Biomedical Journal، جلد ۲۷، شماره ۴، صفحات ۱۶۷-۱۷۲
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عنوان انگلیسی Effect of miR-4270 Suppression on Migration in Hepatocellular Carcinoma Cell Line (HepG2)
چکیده انگلیسی مقاله
Background: Liver transplantation and surgical resection are two major strategies for treatment of hepatocellular carcinoma (HCC) patients. One approach to treating HCC is the suppression of metastasis to other tissues. Herein, we aimed to study the effect of miR-4270 inhibitor on migration of HepG2 cells as well as activity of matrix metalloproteinase (MMP) these cells in order to find a strategy to suppress metastasis in future.
Methods: HepG2 cells were treated with 0, 10, 20, 30, 40, 50, 60, 70, 80, and 90 nM of miR-4270 inhibitor, and then the cell viability was measured by trypan blue staining. Afterwards, cell migration and MMP activity of HepG2 cells were assessed by wound healing assay and zymography, respectively. The MMP gene expression was determined by real-time reverse transcription polymerase chain reaction.
Results: Results showed that miR-4270 inhibitor decreased the cell viability of HepG2 cells in a concentration-dependent manner. Also, inhibition of the miR-4270 reduced invasion, MMP activity, and expression of MMP genes in HepG2 cells, respectively.
Conclusion: Our findings suggest that miR-4270 inhibitor decreases in vitro migration, which could help find a new approach for HCC therapy patients.
کلیدواژه‌های انگلیسی مقاله Hepatocellular carcinoma, Matrix metalloproteinase, Metastasis, MicroRNA
نویسندگان مقاله | Hassan Akrami
Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran


| Hanieh Gholami
Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran


| Mohammad Reza Fattahi
Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran


| Mastaneh Zeraatiannejad
Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-5188-1&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Cancer Biology
نوع مقاله منتشر شده مقاله کامل
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