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Iranian Biomedical Journal، جلد ۲۶، شماره ۵، صفحات ۳۸۰-۳۸۸
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عنوان انگلیسی Evaluation of Anticancer and Cytotoxic Effects of Genistein on PC3 Prostate Cell Line under Three-Dimensional Culture Medium
چکیده انگلیسی مقاله Background: Prostate cancer is a major cause of disease and mortality among men. Genistein (GNT) is an isoflavone found naturally in legumes. Isoflavones, a subset of phytoestrogens, are structurally similar to mammalian estrogens. This study aimed to evaluate the anticancer and cytotoxic effects of GNT on PC3 cell line under three dimensional (3D) culture medium.
Methods: The 3D culture was created by encapsulating the PC3 cells in alginate hydrogel. MTT assay, neutral red uptake, comet assay, and cytochrome C assay were used to study the anticancer and cytotoxic effects of GNT at 120, 240, and 480 μM concentrations. Also, nitric oxide (NO), catalase, and glutathione assay levels were determined to evaluate the effect of GNT on the cellular stress. The culture medium was used as the negative control.
Results: GNT reduced the production of cellular NO and increased the production of catalase and glutathione, confirming the results of the NO test. Evaluation of the toxicity effect of GNT at the concentrations of 120, 240, and 480 μM using comet assay showed that this chemical agent induces apoptosis in PC3 cells in a dose-dependent manner. As the level of cytochrome C in PC3 cells treated with different concentrations of GNT was not significantly different from that of the control, GNT could induce apoptosis in PC3 cells through the non-mitochondrial pathway. 
Conclusion: The findings of this study disclose that the anticancer effect of GNT on PC3 cells under 3D culture conditions could increase the effectiveness of treatment. Also, the cell survival rate is dependent on GNT concentration.
کلیدواژه‌های انگلیسی مقاله Apoptosis, Genistein, Three-dimensional cell culture
نویسندگان مقاله | Seyedeh Masoumeh Khamesi
Department of Medical Radiation Engineering, Central Tehran Branch, Islamic Azad University, Tehran, Iran


| Mehdi Salehi Barough
Department of Medical Radiation Engineering, Central Tehran Branch, Islamic Azad University, Tehran, Iran


| Jamil Zargan
Department of Biology, Faculty of Basic Science, Imam Hossein University, Tehran, Iran


| Mohsen Shayesteh
Department of Physics, Imam Hossein University, Tehran, Iran


| Nooshin Banaee
Department of Medical Radiation Engineering, Central Tehran Branch, Islamic Azad University, Tehran, Iran


| Ashkan Haji Noormohammadi
Department of Biology, Faculty of Basic Science, Imam Hossein University, Tehran, Iran


| Hani Keshavarz Alikhani
Department of Biology, Faculty of Basic Science, Imam Hossein University, Tehran, Iran


| Mohsen Mousavi
Department of Biology, Faculty of Basic Science, Imam Hossein University, Tehran, Iran
نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-4905-1&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Cancer Biology
نوع مقاله منتشر شده مقاله کامل
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