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Iranian Biomedical Journal، جلد ۲۶، شماره ۳، صفحات ۲۳۰-۲۳۹
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چکیده فارسی مقاله
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عنوان انگلیسی Bacterial DNA Detection in the Blood of Healthy Subjects
چکیده انگلیسی مقاله Background: The presence of microbiome in the blood samples of healthy individuals has been addressed. However, no information can be found on the healthy human blood microbiome of Iranian subjects. The current study is thus aimed to investigate the existence of bacteria or bacterial DNA in healthy individuals.
Methods: Blood samples of healthy subjects were incubated in BHI broth at 37 °C for 72 h. The 16S rRNA PCR and sequencing were performed to analyze bacterial isolates. The 16S rRNA PCR was directly carried out on DNA samples extracted from the blood of healthy individuals. Next generation sequencing (NGS) was conducted on blood samples with culture-positive results.
Results: Fifty blood samples were tested, and six samples were positive by culture as confirmed by Gram staining and microscopy. The obtained 16S rRNA sequences of cultured bacterial isolates revealed the presence of Bacilli and Staphylococcus species by clustering in the GeneBank database (≥97% identity). The 16S rRNA gene sequencing results of one non-cultured blood specimen showed the presence of Burkholderia. NGS results illustrated the presence of Romboutsia, Lactobacillus, Streptococcus, Bacteroides, and Staphylococcus in the blood samples of positive cultures.
Conclusion: The dormant blood microbiome of healthy individuals may give the idea that the steady transfer of bacteria into the blood does not necessarily lead to sepsis. However, the origins and identities of blood-associated bacterial rDNA sequences need more evaluation in the healthy population.
 
کلیدواژه‌های انگلیسی مقاله Bacteria, Blood, Microbiome, Sequencing, 16S rRNA
نویسندگان مقاله | Javad Raeisi
Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran


| Mana Oloomi
Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran


| Mohammad Reza Zolfaghari*
Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran


| Seyed Davar Siadat
Department of Mycobacteriology, Pasteur Institute of Iran, Tehran, Iran


| Mohsen Zargar
Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran


| Zahra Pourramezan
Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran
نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-54-4&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Molecular Microbiology
نوع مقاله منتشر شده مقاله کامل
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