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Iranian Biomedical Journal، جلد ۲۶، شماره ۱، صفحات ۷۰-۷۶
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Detection of Microsatellite Instability by High-Resolution Melting Analysis in Colorectal Cancer
چکیده انگلیسی مقاله Background: Colorectal cancer (CRC) is the third most common cancer worldwide. microsatellite instability (MSI) is a molecular marker of a deficient mismatch repair system and happens in almost 15% of CRCs. Because of a wide frequency of MSI+ CRC in Iran compared to other parts of the world, the importance of screening for this type of cancer is highlighted. Methods: The most common MSI detection technique is a fluorescent PCR-based method in which fragments are analyzed by capillary electrophoresis (CE). This technique is very time-consuming, difficult, and expensive. We sought to develop and evaluate a proper method with high accuracy, specificity, and sensitivity to screen the MSI+ CRC. A high-resolution melting (HRM) analysis procedure is relying on the analysis of the melting curve attributes. Low cost, feasibility, high specificity, and sensitivity are outstanding attributes of HRM analysis. Results: Five mononucleotide microsatellite markers, including BAT-25, BAT-26, NR-21, NR-24, and NR-27, in 25 archival CRC tumor tissue samples were compared with normal tissue adjacent using HRM method. The specificity and sensitivity of BAT-25 with HRM method were 100% compared to CE, while other markers had lower sensitivity. However, when all the markers were considered together, the sensitivity and specificity became 100%. The number of MSI+ samples was 56%, which shows a higher ratio than previous Iranian studies. The highest MSI was related to BAT-26 (52%). Conclusion: The HRM method is much simpler and more cost-effective than current MSI techniques, and its sensitivity and accuracy are comparable. Therefore, it can serve as an alternative method in cases where CE is unavailable.
کلیدواژه‌های انگلیسی مقاله Biomarkers, Microsatellite instability, Colorectal cancer
نویسندگان مقاله | Nafiseh Raji
National Institute of Genetic Engineering and Biotechnology


| Tayebeh Majidi Zadeh
National Institute of Genetic Engineering and Biotechnology


| Pegah Babaheidarian
Iran University of Medical Sciences


| Massoud Houshmand
National Institute of Genetic Engineering and Biotechnology
نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-3852-1&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Molecular Genetics & Genomics
نوع مقاله منتشر شده مقاله کامل
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