این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Biomedical Journal، جلد ۲۵، شماره ۴، صفحات ۲۷۵-۲۸۳
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Woodchuck Hepatitis Virus Post-Transcriptional Regulation Element (WPRE) Promotes Anti-CD19 BiTE Expression in Expi293 Cells
چکیده انگلیسی مقاله Background: Bispecific antibodies represent an important class of monoclonal antibodies (mAbs), with great therapeutic potentials due to their ability to target simultaneously two distinct epitopes. The generation of functional bispecific antibodies with the highest possible yields is particularly critical for the production of these compounds on industrial scales. Anti-CD3 × CD19 bispecific antibody (bsAb) is a bispecific T-cell engager currently used for treating ALL. Herein, we have tried to optimize the expression level of this antibody in mammalian hosts.  Methods: Woodchuck hepatitis virus post-transcriptional regulation (WPRE) sequence was incorporated at the 3’ end of the expression cassette. This modification resulted in a notable about two-fold increase in the expression of the bsAb in the Expi293 cell line. Results & Conclusion: Follow-up flow cytometry analysis demonstrated the binding properties of the produced antibody at acceptable levels, and in vitro bioactivity assays showed that this product is potent enough for targeting and destroying CD19-positive cells. Our findings show that WPRE enhances the expression of this type of bispecific mAbs in human embryonic kidney-293 family cell lines. This approach can be used in biopharma industry for the mass production of anti-CD3 × CD19 bispecific antibody.
کلیدواژه‌های انگلیسی مقاله Acute lymphoblastic leukemia, Bispecific antibodies, Monoclonal antibody
نویسندگان مقاله | Reza Moazzami
Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran


| Hasan Mirzahoseini
Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran


| Leila Nematollahi
Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran


| Farzaneh Barkhordari
Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran


| Mozhgan Raigani
Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran


| Fatemeh Hajari Taheri
Department of Immunology, Hybridoma Lab, Pasteur Institute of Iran, Tehran, Iran


| Fereidoun Mahbudi
Department of Immunology, Hybridoma Lab, Pasteur Institute of Iran, Tehran, Iran


| Fatemeh Davami
Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-4244-1&slc_lang=en&sid=1
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Pharmaceutical Biotechnology
نوع مقاله منتشر شده مقاله کامل
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات