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Iranian Biomedical Journal، جلد ۲۵، شماره ۲، صفحات ۷۸-۸۷
عنوان فارسی
چکیده فارسی مقاله
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عنوان انگلیسی Increased Osteogenic Potential of Pre-Osteoblasts on Three-Dimensional Printed Scaffolds Compared to Porous Scaffolds for Bone Regeneration
چکیده انگلیسی مقاله Background: One of the main challenges with conventional scaffold fabrication methods is the inability to control scaffold architecture. Recently, scaffolds with controlled shape and architecture have been fabricated using three-dimensional printing (3DP). Herein, we aimed to determine whether the much tighter control of microstructure of 3DP poly(lactic-co-glycolic) acid/β-tricalcium phosphate (PLGA/β-TCP) scaffolds is more effective in promoting osteogenesis than porous scaffolds produced by solvent casting/porogen leaching. Methods: Physical and mechanical properties of porous and 3DP scaffolds were studied. The response of pre-osteoblasts to the scaffolds was analyzed after 14 days. Results: The 3DP scaffolds had a smoother surface (Ra: 22 ± 3 µm) relative to the highly rough surface of porous scaffolds (Ra: 110 ± 15 µm). Water contact angle was 112 ± 4° on porous and 76 ± 6° on 3DP scaffolds. Porous and 3DP scaffolds had the pore size of 408 ± 90 and 315 ± 17 µm and porosity of 85 ± 5% and 39 ± 7%, respectively. Compressive strength of 3DP scaffolds (4.0 ± 0.3 MPa) was higher than porous scaffolds (1.7 ± 0.2 MPa). Collagenous matrix deposition was similar on both scaffolds. Cells proliferated from day 1 to day 14 by fourfold in porous and by 3.8-fold in 3DP scaffolds. Alkaline phosphatase (ALP) activity was 21-fold higher in 3DP scaffolds than porous scaffolds. Conclusion: The 3DP scaffolds show enhanced mechanical properties and ALP activity compared to porous scaffolds in vitro, suggesting that 3DP PLGA/β-TCP scaffolds are possibly more favorable for bone formation.
کلیدواژه‌های انگلیسی مقاله Alkaline phosphatase, β-tricalcium phosphate, Poly(lactic-co-glycolic) acid copolymer
نویسندگان مقاله | Yasaman Zamani
Department of Biomedical Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran;


| Ghassem Amoabediny
Department of Biomedical Engineering, Research Center for New Technologies in Life Science Engineering, University of Tehran, Tehran, Iran


| Javad Mohammadi
Department of Biomedical Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran;


| Behrouz Zandieh-Doulabi
Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA)-University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam Movement Sciences, Amsterdam, The Netherlands


| Jenneke Klein-Nulend
Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA)-University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam Movement Sciences, Amsterdam, The Netherlands


| Marco N. Helder
Department of Oral and Maxillofacial Surgery/Oral Pathology, Amsterdam University Medical Centers-location VUmc and Academic Centre for Dentistry Amsterdam (ACTA), Amsterdam Movement Sciences, Amsterdam, the Netherlands;
نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-3936-1&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Tissue Engineering and Cell Biology
نوع مقاله منتشر شده مقاله کامل
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