این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Biomedical Journal، جلد ۲۵، شماره ۱، صفحات ۴۱-۴۶
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی In silico Analysis of Pasteurella multocida PlpE Protein Epitopes As Novel Subunit Vaccine Candidates
چکیده انگلیسی مقاله Background: Pasteurella multocida is a Gram-negative, non-motile, non-spore forming, and aerobic/anaerobic cocobacillus known as the causative agent of human and animal diseases. Humans can often be affected by cat scratch or bite, which may lead to soft tissue infections and in rare cases to bacteremia and septicemia. Commercial vaccines against this agent include inactivated, live attenuated, and non-pathogenic bacteria. Current vaccines have certain disadvantages such as reactogenicity or reversion to virulence. Therefore, the aim of this study was to reach a multi-epitope vaccine candidate that could be serotype independent and covers most incident serotypes of P. multocida. Methods: In this study, reverse vaccinology strategy was used to identify potentially immunogenic and protective epitopes. First, multiple alignments of different sequences of Pasteurella lipoprotein E (PlpE) from various serotypes of P. multocida were analyzed to identify the conserved regions. Bioinformatics tools were then applied to predict and select epitopes for further studies. Results: Three different conserved immunogenic regions were selected according to the selected criteria, and their various sequential orders were evaluated structurally by in silico tools to find the best order. Conclusion: In searching the epitopes of PlpE to design a new vaccine candidate against pasteurellosis, we found the region 1 + region 2 + region 3 (without any linker between regions) of epitope, including the regions of PlpE protein of P. multocida, as the appropriate serotype independent vaccine candidate against pasteurellosis.
کلیدواژه‌های انگلیسی مقاله Pasteurella multocida, Polytope, Vaccines
نویسندگان مقاله | Saied Mostaan
Molecular Biology Department, Pasteur Institute of Iran, Tehran, Iran


| Abbas Ghasemzadeh
Molecular Biology Department, Pasteur Institute of Iran, Tehran, Iran


| Parastoo Ehsani
Molecular Biology Department, Pasteur Institute of Iran, Tehran, Iran


| Soroush Sardari
Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran


| Mohammad Ali Shokrgozar
National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran


| Mohsen Abolhassani
Hybridoma Lab, Department of Immunology, Pasteur Institute of Iran, Tehran, Iran


| Gholamreza Nikbakht Brujeni
Department of Microbiology and Immunology, University of Tehran, Iran
نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-837&slc_lang=other&sid=1
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده other
موضوعات مقاله منتشر شده Related Fields
نوع مقاله منتشر شده مقاله کامل
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات