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Iranian Biomedical Journal، جلد ۲۵، شماره ۱، صفحات ۴۷-۵۳
عنوان فارسی
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عنوان انگلیسی OGG1 DNA Repair Gene Polymorphism As a Biomarker of Oxidative and Genotoxic DNA Damage
چکیده انگلیسی مقاله Background: Single nucleotide polymorphisms in 8-oxoguanine DNA glycosylase-1 (OGG1) gene modulates DNA repair capacity and functions as one of the first lines of protective mechanisms against 8-hydroxy-2’-deoxyguanosine (8-OHdG) mutagenicity. OGG1-Cys326 gene polymorphism may decrease DNA repair function, causing oxidative stress due to higher oxidative DNA damage. The main purpose of this study was to examine the link of oxidative and genotoxic DNA damage with DNA repair OGG1 gene polymorphism, in charcoal workers exposed to polyaromatic hydrocarbons. Methods: Urinary 8-OHdG excretion (a biomarker of oxidative DNA damage) was determined in both exposed and control populations. Genotyping of OGG1 DNA repair gene in the blood samples of subjects was carried out by PCR-RFLP method. Results: The 8-OHdG urinary concentration was significantly higher (p < 0.05) in the exposed (geometric mean 12.33 ± 3.78) than in the unexposed (geometric mean 7.36 ± 2.29) population. DNA damage, as measured by 8-OHdG and tail moment content, was found to be significantly higher in OGG1 homozygous mutants (mt/mt; 18.81 ± 3.34; 6.04 ± 0.52) as compared to wild-type genotypes (wt/wt; 10.34 ± 2.25; 5.19 ± 2.50) and heterozygous (wt/mt) mutants (12.82 ± 2.81; 6.04 ± 0.93) in the exposed group. Conclusion: We found a significant association of OGG1 heterozygous (wt/mt) and homozygous (mt/mt) variants with oxidative and genotoxic damage, suggesting that these polymorphisms may modulate the effects of polycyclic aromatic hydrocarbons exposure in occupational workers.
کلیدواژه‌های انگلیسی مقاله 8-hydroxy-2’-deoxyguanosine, 1-hydroxypyrene, Polycyclic aromatic hydrocarbons
نویسندگان مقاله | Kanika Miglani
Department of Biochemistry, Kurukshetra University Kurukshetra, Haryana 136119, India


| Sunil Kumar
Department of Biotechnology, Kurukshetra University Kurukshrtra, Haryana 136119, India


| Anita Yadav
Department of Biotechnology, Kurukshetra University Kurukshrtra, Haryana 136119, India


| Neeraj Aggarwal
Department of Microbiology, Kurukshetra University Kurukshetra, Haryana 136119, India


| Ranjan Gupta
Department of Biochemistry, Kurukshetra University Kurukshetra, Haryana 136119, India
نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-840&slc_lang=en&sid=1
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