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Iranian Biomedical Journal، جلد ۲۴، شماره ۶، صفحات ۳۸۶-۳۹۸
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Status of Biofilm-Forming Genes among Jordanian Nasal Carriers of Methicillin-Sensitive and Methicillin-Resistant Staphylococcus aureus
چکیده انگلیسی مقاله Introduction: Biofilm formation in Staphylococcus aureus is a major virulence factor. Both methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) are common causes of community- and hospital-acquired infections and are associated with biofilm formation. The status of biofilm-forming genes has not been explored in Jordanian nasal carriers of S. aureus. This study investigates antibiotic resistance patterns and the prevalence of biofilm-forming genes between MSSA and MRSA in two distinct populations in Jordan. Methods: A total of 35 MSSA and 22 MRSA isolates were recovered from hospitalized patients and medical students at Prince Hamzah Hospital, Jordan. Antibiotic susceptibility was tested using disk diffusion method and Vitek 2 system. The phenotypic biofilm formation was tested using Congo red agar and microtiter plate assays. The prevalence of the biofilm-forming genes was determined using multiplex PCR. Results: Among 57 S. aureus isolates, 22 (38.6%) isolates were MRSA and were highly resistant against benzylpenicillin, oxacillin, and imipenem. The frequencies of the icaADBC were 77.1%, 97.1%, 94.3%, and 97.1% respectively in MSSA compared to 86.4%, 100%, 100%, and 100% in MRSA isolates. On the other hand, the frequency of the fnbA, fnbB, clfA, fib, clfB, ebps, eno, and cna genes was 81.8%, 90.9%, 95.5%, 90.9%, 86.4%, 100%, 100%, and 40.9%, respectively in the MRSA isolates. Conclusion: In both groups, MRSA isolates, in comparison to MSSA, were significantly more resistant to cefoxitin, oxacillin, imipenem, tetracycline, clindamycin, and trimethoprim-sulfamethoxazole. Unexpectedly, biofilm formation and gene prevalence between MRSA and MSSA isolates showed no significant difference, suggesting other potential virulence mechanisms.
کلیدواژه‌های انگلیسی مقاله Biofilms, Methicillin-resistant Staphylococcus aureus, Methicillin-sensitive Staphylococcus aureus
نویسندگان مقاله | Ashraf I Khasawneh
Department of Basic Medical Sciences, Faculty of Medicine, Hashemite University, Zarqa, Jordan


| Nisreen Himsawi
Department of Basic Medical Sciences, Faculty of Medicine, Hashemite University, Zarqa, Jordan


| Jumana Abu-Raideh
Department of Basic Medical Sciences, Faculty of Medicine, Hashemite University, Zarqa, Jordan


| Muna A. Salameh
Department of Basic Medical Sciences, College of Medicine, Al-Balqa' Applied University, Al-Salt, Jordan


| Mohammad Al-Tamimi
Department of Basic Medical Sciences, Faculty of Medicine, Hashemite University, Zarqa, Jordan


| Sameer Al Haj Mahmoud
Department of Basic Medical Sciences, College of Medicine, Al-Balqa' Applied University, Al-Salt, Jordan


| Tareq Saleh
Department of Basic Medical Sciences, Faculty of Medicine, Hashemite University, Zarqa, Jordan
نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-834&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Molecular Microbiology
نوع مقاله منتشر شده مقاله کامل
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