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Iranian Biomedical Journal، جلد ۲۴، شماره ۶، صفحات ۳۹۹-۴۰۴
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چکیده فارسی مقاله
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عنوان انگلیسی A Real-Time RT-PCR Assay for Genotyping of Rotavirus
چکیده انگلیسی مقاله Background: HRV is the causative agent of severe gastroenteritis in children and responsible for two million hospitalizations and more than a half-million deaths annually. Sequence characteristics of the gene segments encoding the VP7 and VP4 proteins are used for the genotype classification of rotavirus. A wide variety of molecular methods are available, mainly based on reverse transcription PCR for rapid, specific and sensitive genotyping of rotaviruses. This study describes an alternative real-time PCR assay for genotyping of rotavirus. Methods: The samples of stools studied in this research have been collected from patients referred to Children's Medical Centers, Tehran, Iran. Rotavirus detection and genotyping were performed using the RT-PCR and semi-nested RT-PCR, respectively. Samples were then genotyped with a new real-time PCR. Results: The real-time PCR was able to genotype all positive samples with a mean Ct of 28.2. Besides, a concordance rate of 100% was detected between real-time PCR and semi-nested RT-PCR. Conclusion: In this study, the genotyping of rotavirus with real-time PCR showed that this method can provide several favorable features, including high sensitivity and specificity, and a wide dynamic range for rotavirus genotyping.
کلیدواژه‌های انگلیسی مقاله Gastroenteritis, Genotype, Real-time polymerase chain reaction, Rotavirus
نویسندگان مقاله | Seyed Dawood Mousavi-Nasab
Department of Research and Development, Production and Research Complex, Pasteur Institute of Iran, Tehran, Iran


| Farzaneh Sabahi
Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran


| Hooman Kaghazian
Department of Research and Development, Production and Research Complex, Pasteur Institute of Iran, Tehran, Iran


| Mahdi Paryan
Department of Research and Development, Production and Research Complex, Pasteur Institute of Iran, Tehran, Iran


| Siamak Mirab Samiee
Reference Health Laboratories Research Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran


| Mostafa Ghaderi
Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran


| Fatemeh Zali
Department of Clinical Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran


| Manoochehr Makvandi
Infectious and Tropical Diseases Research Center, Health research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-831&slc_lang=other&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده other
موضوعات مقاله منتشر شده Molecular Microbiology
نوع مقاله منتشر شده مقاله کامل
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