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Journal of Medical Microbiology and Infectious Diseases، جلد ۱۲، شماره ۲، صفحات ۱۱۰-۱۲۰
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عنوان انگلیسی Colistin Resistance in WHO-Designated Global Priority Pathogens Isolated from Wastewater Effluents of Two Hospitals in Enugu Metropolis, South East Nigeria
چکیده انگلیسی مقاله Introduction: Hospitals are breeding grounds for multidrug-resistant (MDR) bacteria, posing treatment challenges and increasing the risk of spreading "superbugs." This study investigates the prevalence of colistin-resistant bacteria, a last-resort antibiotic, in wastewater from tertiary hospitals in Enugu, Nigeria. Methods: Twenty wastewater samples were collected over three months from two tertiary hospitals in Enugu. A standardized protocol by the American Public Health Association (APHA) was followed. Samples were collected aseptically from key drainage points and transported to the lab within 2 hours. Bacteria were isolated using the pour-plate method and characterized by morphological and biochemical tests, including Catalase, Oxidase, and Glucose Fermentation. Antibiotic susceptibility was assessed using Kirby-Bauer disc diffusion, and colistin resistance was confirmed via broth microdilution. Multiplex PCR detected mcr genes indicating plasmid-mediated resistance. Data were analyzed using SPSS version 23 with Chi-Square and ANOVA tests at a significance level of P < 0.05. Results: Gram-negative bacteria were isolated from 63.1% of samples, with Klebsiella spp. being the most prevalent, accounting for 24.6%. Colistin resistance was phenotypically observed in E. coli (83%), Klebsiella spp. (75%), and Pseudomonas aeruginosa (100%). Genotypically, E. coli harbored mcr-1 (17%) and mcr-3 (83%), while all Klebsiella and Pseudomonas isolates carried multiple mcr genes. Additionally, these bacteria showed resistance to multiple antibiotics, including Septrin, Gentamycin, and Ceftriaxone. Conclusion: The significant presence of colistin-resistant bacteria, especially E. coli and Klebsiella, poses a public health concern, potentially leading to treatment failures and spreading resistance genes. Stricter monitoring of hospital wastewater is necessary to identify emerging resistance trends and improve antibiotic practices in hospitals.
کلیدواژه‌های انگلیسی مقاله Colistin resistance, Antibiotic resistance, Enterobacteriaceae, Hospital wastewater, mcr-1 gene, mcr-2 gene, mcr-3 gene, Multidrug resistance, Nigeria
نویسندگان مقاله | Oluchi AGBO
Department of Pharmaceutics, University of Nigeria, Nsukka, PMB 410001, Enugu State, Nigeria


| Mumuni Momoh
Department of Pharmaceutics, University of Nigeria, Nsukka, PMB 410001, Enugu State, Nigeria


| Damian Odimegwu
Department of Pharmaceutics, University of Nigeria, Nsukka, PMB 410001, Enugu State, Nigeria


| Cyril Adonu
Department of Pharmaceutical Microbiology and Biotechnology, Enugu State University of Science and Technology, PMB 01660, Enugu State, Nigeria
نشانی اینترنتی http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-588-1&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Anti-microbial agents, resistance and treatment protocols
نوع مقاله منتشر شده Original article
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