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Journal of Medical Microbiology and Infectious Diseases، جلد ۱۲، شماره ۱، صفحات ۵۹-۶۶
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عنوان انگلیسی Dominant EAEC and Widespread Antibiotic Resistance among Diarrheagenic Escherichia coli in Children with Acute Diarrhea in Zahedan, Iran
چکیده انگلیسی مقاله Introduction: Diarrheal disease is a major cause of morbidity and mortality among children under the age of 10 worldwide, particularly in low- and middle-income countries. Diarrheagenic Escherichia coli (DEC) is a common cause of gastroenteritis in children. This study investigated the frequency, virulence markers, and antibiotic resistance patterns of DEC in children below 10 years with acute diarrhea in Zahedan, Iran. Methods: In this cross-sectional study, 300 E. coli isolates were collected from stool samples of children aged below 10 years with diarrhea who presented to hospitals and clinical laboratories in Zahedan. DEC pathotypes were identified using multiplex PCR and confirmed by standard biochemical tests and polyvalent antisera. Results: Of the 300 E. coli isolates examined, 89 (29.6%) were identified as diarrheagenic E. coli (DEC) using polyvalent antisera targeting known DEC pathotypes. Enteroaggregative E. coli (EAEC) was identified in 31 isolates (34.83%) based on reaction with antiserum No. 1. Enterotoxigenic E. coli (ETEC) was identified in 35 isolates (39.33%) based on reaction with antiserum No. 2. Enteropathogenic E. coli (EPEC) was identified in 23 isolates (25.84%) based on reaction with antiserum No. 3 (anti-coli3). Multiplex PCR identified the most common pathotype as EAEC (37.6%), followed by EPEC (21.7%), ETEC (15.9%), and EIEC (11.5%). Statistical analysis revealed no significant correlation between the presence of specific virulence genes (e.g., eae, pcvd432, elt, est, and iaH) and antibiotic resistance patterns in the DEC isolates. Conclusion: Given the distribution of DEC pathotypes among children in Zahedan and their increased antibiotic resistance, antibiotic treatment should be guided by molecular typing and antimicrobial susceptibility testing of isolates, when appropriate.
کلیدواژه‌های انگلیسی مقاله Diarrheagenic E. coli (DEC), Antibiotic resistance, Enteroaggregative E. coli (EAEC), Children, Zahedan, Multiplex PCR
نویسندگان مقاله | Mojdeh Jahantigh
Infectious Diseases and Tropical Medicine Research Center, Research Institute of Cellular and Molecular Sciences in Infectious Diseases, Zahedan University of Medical Sciences, Zahedan, Iran


| Nasrin Seyedzayi
Department of Microbiology, Zahedan University of Medical Sciences, Zahedan, Iran
نشانی اینترنتی http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-483-1&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Epidemiologic studies including microbial genotyping, phenotyping and serotyping
نوع مقاله منتشر شده Original article
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