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Journal of Medical Microbiology and Infectious Diseases، جلد ۱۱، شماره ۳، صفحات ۱۳۶-۱۴۰
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عنوان انگلیسی Isolation and Identification of a Sewage Pseudomonas aeruginosa-infecting Bacteriophage
چکیده انگلیسی مقاله Introduction: Pseudomonas aeruginosa, a nosocomial pathogen, causes respiratory infections, particularly in individuals with cystic fibrosis (CF); it poses a significant challenge due to escalating antibiotic resistance. Phage therapy is emerging as a potential alternative approach to treating antibiotic-resistant bacteria. This study aimed to isolate and identify a highly efficacious bacteriophage targeting P. aeruginosa. Methods: Sewage samples from Mofid Children's Hospital were collected and titrated using a double-layer plaque assay. After plaque detection, the phage was isolated, and then the host range, adsorption rate, and stability were evaluated through spot tests and double-layer plaque assays. Phage morphology was determined using transmission electron microscopy (TEM). Results: Of the three sewage samples collected, one contained phage. We isolated a lytic bacteriophage that exhibited clear plaque formation and induced lysis in planktonic P. aeruginosa. TEM analysis revealed that the phage belonged to the Myoviridae family. The phage had a broad host range and demonstrated optimal stability at 37 °C and pH 7. Conclusion: The isolated phage can potentially be used for treating P. aeruginosa lung infections, offering an alternative approach to address drug-resistant isolates.
 
کلیدواژه‌های انگلیسی مقاله Bacteriophage, Cystic fibrosis, Myoviridae, Phage therapy, Pseudomonas aeruginosa, Antibiotic resistance
نویسندگان مقاله | Mahshid Setayesh Nia
Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran


| Ameneh Elikaei
Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran


| Ahya Abdi Ali
Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran


| Zeynab Rezaei
Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
نشانی اینترنتی http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-496-1&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Anti-microbial agents, resistance and treatment protocols
نوع مقاله منتشر شده Original article
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