این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Journal of Medical Microbiology and Infectious Diseases، جلد ۱۱، شماره ۲، صفحات ۹۶-۱۰۲
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Staphylococcus aureus enterotoxin type B (SEB) and alpha-toxin induced apoptosis in KB cell line
چکیده انگلیسی مقاله Introduction: According to global cancer statistics, oral cancer is the 11th most prevalent cancer worldwide. Despite the availability of numerous modern treatments for oral cancer, a complete reduction of mortality rates has not been achieved. Bacterial toxins have potential applications in inducing apoptosis or targeting tumor cells for destruction. The present study aimed to investigate the impact of Staphylococcus aureus seb and α-toxin genes on apoptotic-related gene mRNA expression, as well as apoptosis induction in KB cell lines, focusing on BAX, RB, BCL-2, and BAG-1 genes. Methods: The transfection of KB cells was performed using Lipofectamine 2000 to introduce pcDNA3.1 (+)-seb, pcDNA3.1 (+)-α-toxin, or an empty pcDNA3.1 (+) plasmid. The cells were cultured in DMEM supplemented with 10% FBS and 800 mg/L of G418 to select cells containing the plasmids. Subsequently, real-time RT-PCR was performed to measure the mRNA expression levels of BAX, RB, BCL-2, and BAG-1 genes. Cell apoptosis was assessed using Annexin V/PI staining and flow cytometry. Results: The seb and α-toxin significantly alter the expression of apoptotic-related genes in the KB cell line. In transfected KB cells, there was a significant increase in the mRNA expression of BAX and RB genes and a substantial decrease in the mRNA expression of BCL-2 and BAG-1 compared to the control group. Annexin test and flow cytometry analysis revealed that seb was more effective than α-toxin in inducing apoptosis. Conclusions: The seb and α-toxin genes of S. aureus exhibit an inhibitory effect on the growth, proliferation, and invasion of oral cancer cells by modulating gene expression in the apoptotic pathway. Hence, these toxins are promising for controlling and treating human oral cancer
کلیدواژه‌های انگلیسی مقاله Staphylococcus aureus, enterotoxin type B, α-toxin, apoptosis, oral cancer, KB cell line, gene expression
نویسندگان مقاله | Milad Heidari
Shahrekord Branch, Islamic Azad University, Biotechnology Research Center, Shahrekord, Iran


| Abbas Doosti
Shahrekord Branch, Islamic Azad University, Biotechnology Research Center, Shahrekord, Iran
نشانی اینترنتی http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-376-3&slc_lang=en&sid=1
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Other
نوع مقاله منتشر شده Original article
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات