این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Journal of Research in Medical Sciences، جلد ۱۸، شماره ۳، صفحات ۲۳۹-۰
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی DNA damage in Iranian veterans 25 years after wartime exposure to sulfur mustard
چکیده انگلیسی مقاله Normal 0 false false false EN-US X-NONE AR-SA Background: More than 100,000 Iranian veterans and civilians still suffer from various long-term complications due to their exposure to sulfur mustard (SM) during the Iran–Iraq war in 1983–88. The aim of the study was to investigate DNA damage of SM in veterans who were exposed to SM, 23–27 years prior to this study. Materials and Methods: Blood samples were obtained from the veterans and healthy volunteers as negative controls. Lymphocytes were isolated from blood samples and DNA breaks were measured using single-cell microgel electrophoresis technique under alkaline conditions (comet assay). Single cells were analyzed with “Tri Tek Comet Score version 1.5” software and DNA break was measured based on the percentage of tail DNA alone, or in the presence of H 2 O 2 (25 μM) as a positive control. Results: A total of 25 SM exposed male veterans and 25 male healthy volunteers with similar ages (44.66 ± 6.2 and 42.12 ± 5.75 years, respectively) were studied. Percentage of the lymphocyte DNA damage was significantly ( p < 0.01) higher in the SM-exposed individuals than in the controls (6.47 ± 0.52 and 1.31 ± 0.35, respectively). Percentages of DNA damage in the different age groups of 35–39, 40–44, 45–49, and 50–54 years in SM-exposed veterans (5.48 ± 0.17, 6.7 3 ± 1.58, 6.42 ± 0.22, and 7.27 ± 0.38, respectively) were all significantly ( p < 0.05) higher than the controls (1.18 ± 0.25, 1.53 ± 0.22, 1.27 ± 0.20, and 1.42 ± 0.10, respectively). The lymphocytes incubated with H 2 O 2 had much higher DNA damage as expected. The average of tail DNA is 42.12 ± 2.75% for control cells + H 2 O 2 and 18.48 ± 2.14% for patients cells + H 2 O 2 ; P < 0.001. Conclusion: SM exposure of the veterans revealed DNA damage as judged by the comet assay.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله عفت بهروان | effat behravan
medical toxicology research center, faculty of medicine and department of pharmacodynamics and toxicology, school of pharmacy, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

سید عادل معلم | seyed adel moallem
department of pharmacodynamics and toxicology, school of pharmacy and pharmaceutical sciences research center, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

شهریار خاطری | shahriar khateri
janbazan medical and engineering research center, janbazan foundation, tehran, iran


الهام مراغی | elham maraghi
department of epidemiology and biostatistics, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

پاوول jowsey | paul jowsey
medical toxicology centre, wolfson building, newcastle university, newcastle upon tyne, united kingdom


پیتر g blain | peter g blain


مهدی بلالی مود | mahdi balali mood
medical toxicology research center, faculty of medicine, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

نشانی اینترنتی http://jrms.mui.ac.ir/index.php/jrms/article/view/9010
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/218/article-218-330991.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Articles
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات