| چکیده انگلیسی مقاله |
Spinal cord injury (SCI) in humans remains a devastating and incurable disorder. A very important obstacle in axonal regeneration after spinal cord injury is astroglial scaring. The use of self-assembling peptide nanofiber, a hydrogel mimicking extracellular matrix, has been suggested as a scaffold for spinal cord regeneration and inhibition of astrogliosis. However, neurogenesis potential of laminim has been proved. The purpose of this study was to investigate the role of self-assembling peptide nanofiber containing long motif of laminin (SAPN-LL) in neural differentiation of human endometrial-derived stem cells (hEnSCs) in-vitro, in polymerization of tubulin isolated from sheep brain ex-vivo and assess the supportive effects of this hydrogel in an animal model of SCI. Results showed that although nanaofibers strongly differentiated hEnSC towards neuron and there were not significant differences between their neural differentiations but motor recovery results demonstrated that concentration of laminin influences motor recovery and tubulin polymerization. However, both of in-vitro and in-vivo results showed that SAPN-LL inhibited astrogenesis. Based on our results it might be concluded that, SAPN containing long motif of laminin holds great promise for spinal cord injury. |
| نویسندگان مقاله |
شیما توکل | shima tavakol
a. department of medical nanotechnology, school of advanced technologies in medicine, tehran university of medical sciences, tehran, iran. b. razi drug research center, iran university of medical sciences, tehran, iran. c. student’s scientific research center, tehran university of medical sciences, tehran, iran. d. shefa neuroscience research center, khatam alanbia hospital, tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
رضا صابر | reza saber
department of medical nanotechnology, school of advanced technologies in medicine, tehran university of medical sciences, tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
الهام حویزی | elham hoveizi
department of biology, faculty of sciences, shahid chamran university of ahvaz, ahvaz, iran.
سازمان اصلی تایید شده: دانشگاه شهید چمران (Shahid chamran university)
هادی علیقلی | hadi aligholi
a. shefa neuroscience research center, khatam alanbia hospital, tehran, iran. b. department of neurosciences, school of advanced technologies in medicine, tehran university of medical sciences, tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
جعفر ai | jafar ai
a. department of tissue engineering, school of advanced technologies in medicine, tehran university of medical sciences, tehran, iran. b. brain and spinal injury research center, imam hospital, tehran university of medical sciences, tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
مهدی رضایت | mahdi rezayat
a. department of medical nanotechnology, school of advanced technologies in medicine, tehran university of medical sciences, tehran, iran. b. department of toxicology amp;amp; pharmacology, school of pharmacy, pharmaceutical sciences branch, islamic azad university iaups , tehran, iran. c. department of pharmacology, school of medicine, tehran university of medical sciences, tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
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