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مجله علوم اعصاب شفای خاتم، جلد ۲، شماره ۴، صفحات ۱۲۴-۱۲۴
عنوان فارسی P۷۴: Differentiation of Bone Marrow Stem Cells (BMSC) to Neurosphere Using Bioactive Substance ATC
چکیده فارسی مقاله لطفاً به چکیده انگلیسی مراجعه شود.
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی P74: Differentiation of Bone Marrow Stem Cells (BMSC) to Neurosphere Using Bioactive Substance ATC
چکیده انگلیسی مقاله Generation neural stem cells from neurosphere–derived bone marrow stem cells using bioactive substance ATC. Bone marrow cells (BMSCs) were isolated from rat. BMSCs cultured by DMEM/F12 medium supplemented with 10% fetal bovine serum. These cells evaluated by specific markers of BMSCs such as bioactive substance ATC, B27. Then BMSCs differentionated in to neurosphere and divided in two groups which were evaluated morphologically. Diameter and number of this neurosphere evaluated daily. BMSCs markers were measured by immunocytochemistery that expressed, CD 90 (75%) CD 44 (60%) fibronectin. Diameter and number of Neurosphere by 0.1 ng/ml was optimal dose for expansion. Then these cells evaluated by neuroectodermal markers such as nestin and NF 68, NF 200 and NF160, that expressed >80 % and this data approved by RT-PCR assay. This study develops a simplified, efficient, and nontoxic approach by lowest factors which derives a large number of neurospheres from BMSCs. With our newly devised approach 10 to 15 passage cells were used for in vitro differentiation. Neuronal differentiation was induced by incubation of the BMSCs with bioactive substance (ATC) induction media.
کلیدواژه‌های انگلیسی مقاله Bone Marrow Cells, Bioactive Substance ATC, Cell Therapy
نویسندگان مقاله لیلا علیزاده | leila alizadeh
shefa neuroscience research center, khatam alanbia hospital, tehran, iran.

سازمان اصلی تایید شده: مرکز تحقیقات علوم اعصاب شفا (Shefa neuroscience research center)

نشانی اینترنتی http://shefayekhatam.ir/browse.php?a_code=A-10-24-472&slc_lang=fa&sid=fa
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/337/article-337-332321.pdf
کد مقاله (doi)
زبان مقاله منتشر شده fa
موضوعات مقاله منتشر شده تحقیقات پایه در علوم اعصاب
نوع مقاله منتشر شده پژوهشی
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