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Journal of Medical Microbiology and Infectious Diseases، جلد ۳، شماره ۱، صفحات ۲۳-۲۸
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چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی A Comparison between Culture and Multiplex PCR for Detection and Identification of Shigella Species in Patients with Shigellosis from Isfahan Province in 2014-2015
چکیده انگلیسی مقاله Introduction: Awareness of the species of Shigella has particular importance in the way of dealing with an outbreak, controlling it, and treating patients. The purpose of this study was to use the Multiplex PCR method and comparison with the culture method to detect Shigella species, as well as analysing the antibiotic resistance pattern in its different species. Methods: Simultaneously, the fecal samples of the patients with diarrhea and a sample from each patient in Cary-Blair medium were sent to the laboratory. Cultivation, serotyping, and antibiogram analysis were performed using the samples inoculated in the Cary-Blair medium. DNA extraction of the fecal samples and amplifying of invC, rfc, wbgZ, and rfpB genes were performed. Results: Totally, 300 samples from the patients were analyzed, out of which 240 Shigella isolated (80%) were detected using the culture method and serotyping, and 260 Shigella isolated (86.6%) using the PCR method (Shigella flexneri (77%), Shigella sonnei (19.2%), and Shigella dysenteriae (3.8%)). 20 samples suspicious of Shigella were observed in the culture method but were not identified by serotyping. However, in the PCR assay, they were identified as S. flexneri (n=16) and S. sonnei (n=4). The resistance of Shigella isolated to Co-trimoxazole was observed to be (78.3%), Ceftriaxone (42.5%), Cefixime (42%), Azithromycin (40.7%), Ofloxacin (34.5%), Nalidixic acid (25 %), and Ciprofloxacin (16%). Conclusion: Due to annual outbreaks of various Shigella species in the country, it is recommended that the Multiplex PCR method, be used along with culture method in laboratories to identify Shigella isolated. The antibiogram results showed increasing resistance of Shigella to the available antibiotics.
کلیدواژه‌های انگلیسی مقاله Shigella, Diarrhea, Antibiotics, Multiplex PCR.
نویسندگان مقاله اعظم فتاحی | azam fatahi
ibn sina street,isfahan,isfahan health center


علی عجمی | ali ajami
ibn sina street,isfahan,isfahan health center


مژگان بزرگ زاد | mojgan bozorgzad
ibn sina street,isfahan,isfahan health center


شهرام نکوییان | shahram nekoeian
ibn sina street,isfahan,isfahan health center


عاطفه خازنی | atefeh khazeni
ibn sina street,isfahan,isfahan health center
نشانی اینترنتی http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-101-1&slc_lang=en&sid=en
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/350/article-350-332412.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Microbial pathogenesis
نوع مقاله منتشر شده Original article
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