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Molecular Biology Research Communications، جلد ۶، شماره ۱، صفحات ۳۳-۴۰
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عنوان انگلیسی Replacement of threonine-55 with glycine decreases the reduction rate of OsTrx20 by glutathione
چکیده انگلیسی مقاله Thioredoxins (Trxs) are small ubiquitous oxidoreductase proteins with two redox-active Cys residues in a conserved active site (WCG/PPC) that regulate numerous target proteins via thiol/disulfide exchanges in the cells of prokaryotes and eukaryotes. The isoforms OsTrx23 with a typical active site (WCGPC) and OsTrx20 with an atypical active site (WCTPC) are two Trx h- type isoforms in rice that were previously found to be reduced by NADPH-dependent thioredoxin reductase and GSH/Grx system, respectively. In the present work the reduction of mutants G41TOsTrx23, T55GOsTrx20, K48DOsTrx20 and T55G-K48D OsTrx20 as well as wild types OsTrx23 and OsTrx20 were tested in the reaction containing either NADPH/NTR or glutathione (GSH). The results revealed that reduction rate of T55GOsTrx20 was remarkably decreased by GSH as compared to WtOsTrx20 highlighting the critical role of Thr-55 in interaction of OsTrx20 with GSH. On the other hand a significant decrease in the reduction rate of G41TOsTrx23 was observed in reaction containing NADPH-dependent thioredoxin reductase as compared with readuction rate of WtOsTrx23. These results suggest that first residue after N-terminal active site Cys is one of the critical residue in determination of system that Trxs can be reduced in.
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نویسندگان مقاله میترا رودگر |
department of biotechnology, college of agriculture, isfahan university of ‎technology, isfahan, iran.‎
سازمان اصلی تایید شده: دانشگاه اصفهان (Isfahan university)

آذر شاهپیری |
department of biotechnology, college of agriculture, isfahan university of ‎technology, isfahan, iran.‎
سازمان اصلی تایید شده: دانشگاه اصفهان (Isfahan university)

نشانی اینترنتی http://mbrc.shirazu.ac.ir/article_4001_75fa106c25b9058b474a027e68e26a5f.pdf
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