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Iranian Journal of Microbiology، جلد ۱۶، شماره ۶، صفحات ۷۶۳-۷۷۲
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عنوان انگلیسی Anti-invasion activities of heat-killed lactic acid bacteria isolates against Salmonella enterica serovar Typhimurium
چکیده انگلیسی مقاله Background and Objectives: The most common cause of severe foodborne salmonellosis is S. Typhimurium. Its interaction with intestinal epithelial cells is little known. Lactic acid bacteria (LAB) were recognized as a prominent probiotic gastrointestinal microbiota of humans and animals that confer health-promoting and protective effects. This study aims to determine the anti-invasion and antibacterial effects of heat-killed LAB (HK-LAB) isolates against S. Typhimurium towards human intestinal cells. Materials and Methods: 12 HK-LAB isolates from 3 sources of origin (stingless bee, plant, and food) were tested to determine the adhesion of HK-LAB to Caco-2 cells, anti-invasion and antibacterial activities against S. Typhimurium, the adhesion and invasion pattern of S. Typhimurium on intestinal epithelial cells (Caco-2) and assessing the effect of LAB on the S. Typhimurium-host cell interaction. Results: Tairu isolates from food have the highest adhesion rate with 19 ± 1.32/10 Caco-2 cells followed by HK-LAB R-isolate from plant 17 ± 0.70/10 Caco-2 cells, which is similar to the control (Lactobacillus casei). In the anti-invasion assay, the two HK-LAB isolates that had the strongest adherence to Caco-2 cells, Tairu-isolate inhibited at 78.1 ± 3.06% and R-isolate inhibited at 64.76 ± 9.02% compared to the positive control (63.81 ± 1.15%), which led to increased suppression of S. Typhimurium accordingly. Tairu and R isolates were tested for their antibacterial ability against S. Typhimurium. Both R and Tairu isolates displayed strong inhibition zones (27 ± 0.06 mm, 23 ± 0.06 mm) respectively. Conclusion: These findings suggest that the anti-invasion activities of HK-LAB R and Tairu may correlate to their bactericidal effects that serve to protect the host from infection.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | Anis Syahirah Saifor Adzuan
Microbial Metabolite Laboratory, Atta-ur-Rahman Institute for Natural Products Discovery (AuRIns), Universiti Teknologi MARA (UiTM), Puncak Alam, Selangor, Malaysia; School of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), Shah Alam, Selangor, Malaysia


| Sharifah Aminah Syed Mohamad
Microbial Metabolite Laboratory, Atta-ur-Rahman Institute for Natural Products Discovery (AuRIns), Universiti Teknologi MARA (UiTM), Puncak Alam, Selangor, Malaysia; School of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), Shah Alam, Selangor, Malaysia


| Rashidah Iberahim
School of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), Kuala Pilah, Negeri Sembilan, Malaysia


| Noor Nadia Syahira Mohd Kamal
School of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), Shah Alam, Selangor, Malaysia


| Nurliana Abd Mutalib
Microbial Metabolite Laboratory, Atta-ur-Rahman Institute for Natural Products Discovery (AuRIns), Universiti Teknologi MARA (UiTM), Puncak Alam, Selangor, Malaysia


| Nur Intan Hasbullah
Microbial Metabolite Laboratory, Atta-ur-Rahman Institute for Natural Products Discovery (AuRIns), Universiti Teknologi MARA (UiTM), Puncak Alam, Selangor, Malaysia; School of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), Kuala Pilah, Negeri Sembilan, Malaysia


| Muneer Alsaydi
Microbial Metabolite Laboratory, Atta-ur-Rahman Institute for Natural Products Discovery (AuRIns), Universiti Teknologi MARA (UiTM), Puncak Alam, Selangor, Malaysia; Department of Food Science and Technology, Faculty of Agriculture, Ibb University, Ibb, Yemen


| Nor'aishah Hasan
School of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), Kuala Pilah, Negeri Sembilan, Malaysia


| Low Kheng Oon
Cell and Synthetic Biology Centre, Malaysia Genome and Vaccine Institute (MGVI), National Institutes of Biotechnology Malaysia (NIBM), Kajang, Selangor, Malaysia


| Olaide Olawunmi Ajibola
Faculty of Resource Science and Technology, Universiti Malaysia Sarawak (UNIMAS), Kota Samarahan, Sarawak, Malaysia


| Rozila Alias
Centre for Foundation and General Studies, Universiti Selangor, Shah Alam, Selangor, Malaysia


| Maimunah Mustakim
Centre for Medical Laboratory Technology Studies, Faculty of Health Sciences, Universiti Teknologi MARA (UiTM), Puncak Alam, Selangor, Malaysia


| Azlin Sham Rambely
Centre for Medical Laboratory Technology Studies, Faculty of Health Sciences, Universiti Teknologi MARA (UiTM), Puncak Alam, Selangor, Malaysia


| Emida Mohamed
Centre for Medical Laboratory Technology Studies, Faculty of Health Sciences, Universiti Teknologi MARA (UiTM), Puncak Alam, Selangor, Malaysia


| Mohammad Reza Pourmand
Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
نشانی اینترنتی https://ijm.tums.ac.ir/index.php/ijm/article/view/4746
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