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Iranian Journal of Basic Medical Sciences، جلد ۲۸، شماره ۲، صفحات ۱۸۷-۱۹۳
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عنوان انگلیسی Puerarin alleviates renal ischemia/reperfusion injury by inhibiting apoptosis and endoplasmic reticulum stress via Nrf2/HO-1 pathway
چکیده انگلیسی مقاله Objective(s): To explore the effects of puerarin on renal ischemia/reperfusion injury and the possible mechanism.Materials and Methods: The experimental mice were injected with puerarin (50 or 100 mg/kg) per day or equal sterile saline by intraperitoneal injection for one week, and a renal I/R injury model was constructed. HK-2 cells were incubated with puerarin (1 uM and 10 uM) before the H/R model. Immunohistochemistry, immunocytochemistry, and Western blot analysis were used to detect the protein associated with apoptosis and endoplasmic reticulum stress.Results: Puerarin could improve renal function and attenuate tissue structural damage after renal I/R. Meanwhile, puerarin alleviated apoptosis and endoplasmic reticulum stress by decreasing expression levels of specific biomarkers such as caspase-3, GRP78, CHOP, and p-elF2α/ elF2α in animals and HK-2 cells. The up-regulated expression of Nrf2 and HO-1 protein after puerarin treatment indicated that the Nrf2/HO-1 signaling pathway might mediate the protective mechanism of puerarin against renal I/R.Conclusion: Our results suggest that puerarin alleviated renal ischemia/reperfusion injury by inhibiting apoptosis and endoplasmic reticulum stress via the Nrf2/HO-1 pathway and offered new insights for preventing and treating renal I/R.
کلیدواژه‌های انگلیسی مقاله Apoptosis, Endoplasmic reticulum- stress, Nrf2/HO-1 pathway, Puerarin, Renal ischemia/reperfusion- injury
نویسندگان مقاله | Jingsong Wang
Department of Urology, Renmin Hospital of Wuhan University, Wuhan, Hubei, 430060, China|Institute of Urologic Disease, Renmin Hospital of Wuhan University, Wuhan, Hubei, 430060, China


| Qingyuan Zheng
Department of Urology, Renmin Hospital of Wuhan University, Wuhan, Hubei, 430060, China|Institute of Urologic Disease, Renmin Hospital of Wuhan University, Wuhan, Hubei, 430060, China


| Jun Jian
Department of Urology, Renmin Hospital of Wuhan University, Wuhan, Hubei, 430060, China|Institute of Urologic Disease, Renmin Hospital of Wuhan University, Wuhan, Hubei, 430060, China


| Xiuheng Liu
Department of Urology, Renmin Hospital of Wuhan University, Wuhan, Hubei, 430060, China|Institute of Urologic Disease, Renmin Hospital of Wuhan University, Wuhan, Hubei, 430060, China


| Zhiyuan Chen
Department of Urology, Renmin Hospital of Wuhan University, Wuhan, Hubei, 430060, China|Institute of Urologic Disease, Renmin Hospital of Wuhan University, Wuhan, Hubei, 430060, China


| Shanshan Wan
Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan, Hubei, 430060, China


| Lei Wang
Department of Urology, Renmin Hospital of Wuhan University, Wuhan, Hubei, 430060, China|Institute of Urologic Disease, Renmin Hospital of Wuhan University, Wuhan, Hubei, 430060, China
نشانی اینترنتی https://ijbms.mums.ac.ir/article_25144.html
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