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Research in Pharmaceutical Sciences، جلد ۱۹، شماره ۶، صفحات ۷۴۶-۷۵۳
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چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Antiaging properties of chlorogenic acid through protein and gene biomarkers in human skin fibroblast cells as photoaging model
چکیده انگلیسی مقاله Background and purpose : Chlorogenic acid (CA) is a natural chemical that promises antiaging activity against photoaging skin damage. This research examined CA activities in mitigating skin photoaging. Experimental approach: UV-exposed human skin fibroblast cells were subjected to CA at 6.25, 12.5, and               25 μg/mL. The protein levels of cell secretion, such as cyclooxygenase (COX)-2, nitric oxide (NO), and interleukin (IL)-6 were measured using ELISA and colorimetry methods. Meanwhile, the mRNA expressions of glutathione peroxidase (GPX)-1, tissue inhibitor metalloproteinase (TIMP)-1, matrix metalloproteinase (MMP)-1, caspase (CASP)-3, CASP-8, and fibroblast growth factor (FGF)-2 were quantified using the qRT-PCR method.  Findings/Results: CA treatment reduced inflammatory and aging biomarkers. CA at 6.25 μg/mL lowered NO, COX-2, and IL-6 levels to 89.44 μmol/L, 8.10 ng/mL, and 62.75 pg/mL, respectively. CA at 25 μg/mL resulted in the most significant down-regulation of MMP-1, CASP-3, and CASP-8 genes’ expression (3.27, 1.25, and 3.59, respectively). Furthermore, treatment with CA at 25 µg/mL demonstrated the most notable activity in up-regulating antioxidant markers, specifically GPX-1, and extracellular matrix (ECM) integrity markers, including TIMP-1 and FGF-2 genes’ expression. Conclusion: CA imposes its anti-aging activity by decreasing inflammatory and aging biomarkers, and increasing cellular antioxidant and ECM integrity.    
کلیدواژه‌های انگلیسی مقاله Antiaging,Chlorogenic acid,Fibroblast,qRT-PCR,Ultraviolet.
نویسندگان مقاله | Ermi Girsang
Faculty of Medicine, Universitas Prima Indonesia, Jl. Sampul No. 4, Medan 20118, North Sumatera, Indonesia.


| Chrismis N. Ginting
Faculty of Medicine, Universitas Prima Indonesia, Jl. Sampul No. 4, Medan 20118, North Sumatera, Indonesia.


| I Nyoman Ehrich Lister
Faculty of Medicine, Maranatha Christian University, Jl. Surya Sumantri No. 65, Bandung 40164, West Java, Indonesia.


| Wahyu Widowati
Biomolecular and Biomedical Research Center, Aretha Medika Utama, Babakan Jeruk II No. 9, Bandung 40163, West Java, Indonesia.


| Afif Yati
Biomolecular and Biomedical Research Center, Aretha Medika Utama, Babakan Jeruk II No. 9, Bandung 40163, West Java, Indonesia.


| Hanna Sari Widya Kusuma
Biomolecular and Biomedical Research Center, Aretha Medika Utama, Babakan Jeruk II No. 9, Bandung 40163, West Java, Indonesia. Biomedical Engineering, Department of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, West Java, Indonesia.


| Rizal Azis


نشانی اینترنتی http://rps.mui.ac.ir/index.php/jrps/article/view/2285
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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