A sustainable entrepreneurial ecosystem is developed and expanded with an emphasis on innovation-based entrepreneurship in emerging Start-ups. This phenomenon requires identifying the influencing factors in this process. This study aimed to analyze the agricultural start-up ecosystem in order to provide a model of the entrepreneurship in Iran. A survey research method was applied to achieve research objectives. The population of the study consisted of managers of agricultural Start-up of Iran, (N= 90). The questionnaire was the main instrument to collect data. Confirmatory factor analysis and structural equation modeling were used to analyze the data. In total, the research results showed that 9 constructs and 131 sub-constructs could explain 82.1% of the variance of entrepreneurial sustainability in agricultural Start-ups. Based on the obtained results, the overall goodness of fit statistics shows that the structural model fits well with the data. The planners of the agricultural entrepreneurship sector can play an effective role in smoothing the development path of agricultural entrepreneurship in Start-ups by using the results of this study and considering the identified factors.