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Journal of Medical Microbiology and Infectious Diseases، جلد ۱۲، شماره ۳، صفحات ۲۰۱-۲۰۷
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عنوان انگلیسی Differential Immunostimulatory Effects of EGFP and +36 GFP on Immune Cells
چکیده انگلیسی مقاله Introduction: Green fluorescent protein (GFP) and its variants are pivotal in tracking gene expression across various gene delivery systems. While GFP is typically employed for intracellular reporting, it can be modified to display on cell surfaces for labeling. Previous research indicates GFP might have immunogenic effects, notably enhancing tumor-specific T cell responses. This study explores the immunostimulatory differences between enhanced GFP (EGFP) and the supercharged variant, +36 GFP. Methods: Recombinant EGFP and +36 GFP proteins were generated using an Escherichia coli expression system. Murine bone marrow-derived dendritic cells (BMDCs) were generated using established protocols. Splenocytes were isolated from murine spleens via mechanical disruption and red blood cell lysis. The RAW 264.7 macrophage cell line was cultured in complete DMEM medium. Immune cells were then incubated with varying concentrations of EGFP and +36 GFP, separately, for 48 h. Cytokine levels (IFN-γ, TNF-α, IL-10) were quantified using sandwich ELISA.
کلیدواژه‌های انگلیسی مقاله Green Fluorescent Protein,Enhanced GFP,Supercharged +36 GFP,Immunogenicity,Cytokines
نویسندگان مقاله | Parisa Moradi Pordanjani
Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran;


| Azam Bolhassani
Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran


| Elnaz Agi
Blood Diseases Research Center (BDRC), Iranian Comprehensive Hemophilia Care Center, Iran University of Medical Sciences (IUMS), Tehran, Iran
نشانی اینترنتی http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-441-4&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Immune responses, deficiencies and vaccine candidates
نوع مقاله منتشر شده Original article
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