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Iranian Journal of Basic Medical Sciences، جلد ۲۸، شماره ۳، صفحات ۳۴۰-۳۴۶
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عنوان انگلیسی COTI-2 suppresses the malignancy of bladder cancer by inducing apoptosis via the AMPK-mTOR signaling pathway
چکیده انگلیسی مقاله Objective(s): COTI-2, an innovative oral homocysteine, has shown promising antitumor results on multiple types of cancer. However, its effects in treating bladder cancer (BCa) and the underlying molecular mechanisms have not been elucidated. The present study aimed to explore the antitumor effects of COTI-2 on BCa and the potential mechanisms.Materials and Methods: BCa cell lines, including the 5637 and T24 cell lines, were treated with COTI-2 at concentrations of 0.5 and 1 μM, respectively. Cell Counting Kit (CCK)-8 assay, colony formation assay, apoptosis assay, and transwell migration and invasion assay were conducted to evaluate the antitumor effects of COTI-2 on BCa cells. Western blotting, H&E, immunohistochemical staining, and immunofluorescence analysis were performed to investigate the underlying mechanisms. Moreover, a xenograft model in nude mice using T24 cells was generated to determine the antitumor activities of COTI-2 in vivo.Results: COTI-2 highly inhibited the proliferation of BCa cell lines, including 5637 and T24 cells, and induced their apoptosis. Moreover, it efficiently suppressed the migration and invasion of BCa cells. Additionally, the subcutaneous xenograft model in nude mice showed that COTI-2 treatment inhibited the tumor growth of BCa by inducing its apoptosis in vivo. We also found that COTI-2 promoted apoptosis in BCa cells, presumably through activating the AMPK/mTOR pathway.Conclusion: Our data suggest that COTI-2 effectively reduces the malignancy of BCa, probably by inducing apoptosis via the AMPK/mTOR signaling pathway. These data highlight the potential of COTI-2 as a therapeutic agent for the treatment of BCa. 
کلیدواژه‌های انگلیسی مقاله Apoptosis, AMPK/mTOR pathway, Bladder cancer, COTI-2, Proliferation
نویسندگان مقاله | Yuancai Zheng
Department of Urology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, 310000, China|Department of Urology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China


| Keqi Wang
Department of Pediatrics, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China


| Chenyu Wu
Department of Pediatrics, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China


| Yuying Qin
Department of Pediatrics, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China


| Yihan Sun
The Second Clinical Medical College of Wenzhou Medical University, Wenzhou, 325000, China


| Xinyu Lu
The First Clinical Medical College of Wenzhou Medical University, Wenzhou, 325000, China


| Yupeng Xu
The First Clinical Medical College of Wenzhou Medical University, Wenzhou, 325000, China


| Gonghui Li
Department of Urology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, 310000, China
نشانی اینترنتی https://ijbms.mums.ac.ir/article_25264.html
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