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Iranian Journal of Basic Medical Sciences، جلد ۲۸، شماره ۴، صفحات ۴۹۸-۵۰۶
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عنوان انگلیسی In vitro and in vivo evaluation of photo-induced antileishmanial activity of indocyanine green-loaded nanomicelles
چکیده انگلیسی مقاله Objective(s): Due to its low toxicity and high absorbance in the range of 650 to 900 nm, indocyanine green (ICG) has garnered significant attention for its applications in photodynamic therapy (PDT) and photothermal therapy (PTT). However, its tendency to aggregate in aqueous environments limits its efficacy in both in vitro and in vivo applications. Encapsulating ICG in a biocompatible nanomicelle can improve its aqueous stability and photophysical properties. The present study investigated the synergistic effect of ICG-loaded nanomicelles upon irradiation by an 808-nm laser on Leishmania major (L. major) parasites. Materials and Methods: Initially, a nanomicelle comprised ICG was synthesized and characterized. Then, the temperature increase during irradiation and promastigote viability were evaluated in vitro. Subsequently, the prepared samples’ in vitro dark toxicity and phototoxicity were assessed via the MTS assay. Finally, the in vivo antileishmanial efficacy of the ICG-loaded nanomicelles formulation was investigated in BALB/c mice. Results: The absorbance of ICG-loaded nanomicelles at 808 nm was more than 2 times greater than Free-ICG. Also, the prepared formulation exhibited a mean diameter of ~25 nm and a zeta potential of -2.3 ± 1 mV. The combination of ICG-loaded nanomicelles and 808 nm laser with a power density of 2.5 W cm−2 led to a significant reduction in the survival rate of promastigotes and lesion size of infected mice compared to control groups. Conclusion: The PDT/PTT mediated by ICG-loaded nanomicelles can be considered a promising and efficient therapeutic method for L. major, as it is inexpensive, safe, and easy to implement.
کلیدواژه‌های انگلیسی مقاله Leishmania major, Nanomedicine, Photodynamic Therapy (PDT), Photosensitizer, Photothermal therapy (PTT)
نویسندگان مقاله | Shirin Jalili
Institute of Police Equipment and Technologies, Policing Sciences and Social Studies Research Institute, Tehran, Iran


| Jafar Mosafer
Research Center of Advanced Technologies in Medicine, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran|Health Sciences Research Center, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran


| Seyed Amin Mousavinezhad
Research Center for Life & Health Sciences & Biotechnology of the Police, Directorate of Health, Rescue & Treatment, Police Headquarters, Tehran, Iran


| Ameneh Sazgarnia
Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran


| Mohammad Ali Mohaghegh
Health Sciences Research Center, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran|Department of Laboratory Sciences, School of Paramedical Sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran


| Mehdi Hoseini
Department of Radiology Technology, School of Paramedical Sciences, Torbat Heydarieh University of Medical Sciences, Torbat Heydarieh, Iran
نشانی اینترنتی https://ijbms.mums.ac.ir/article_25489.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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