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Iranian Journal of Blood and Cancer، جلد ۱۶، شماره ۴، صفحات ۳۰-۳۸
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عنوان انگلیسی Flow Cytometry-based Functional Assay is a Valuable Diagnostic Approach for Confirmation of Heparin-Induced Thrombocytopenia
چکیده انگلیسی مقاله Background: Heparin-induced thrombocytopenia (HIT) is a serious immunological adverse drug reaction that rarely occurs in patients receiving heparin. The heparin-induced platelet activation (HIPA) test, a gold-standard assay for HIT, is time-consuming, challenging, and produces qualitative results. We aimed to compare the performance properties of a flow cytometry-based functional assay for HIT diagnosis with HIPA assay.
Materials and Methods: This research was carried out on HIT-suspected patients referred to the Iranian Blood Transfusion Organization between 2021 and 2023. After clinical evaluation and 4Ts scores calculation, anti-PF4 screening and HIPA test were conducted. Thirty HIPA-positive and 30 HIPA-negative samples were selected. Subsequently, a flow cytometry-based functional assay, Emo-Test HIT confirm, was performed, and the sensitivity and specificity for HIT diagnosis were measured.
Results: Among the 30 samples with negative HIPA results, one was positive with the Emo-test HIT Confirm® assay, and the remaining were negative. Among 30 positive HIPA samples, the result of one sample was inconclusive, two samples were negative with flowcytometry Emo-test and the others were positive. The sensitivity and specificity of this flow cytometry-based functional assay were 90% (95% CI: 79.3-100) and 96.6% (95% CI:90.2-100). The negative predictive value and positive predictive value were 93.5% and 96.4% respectively.
Conclusion: Flow cytometry-based functional assay has a good sensitivity and specificity for HIT diagnosis confirmation, indicating that it may be a promising approach in the clinical setting.
کلیدواژه‌های انگلیسی مقاله Heparin-induced thrombocytopenia (HIT), Diagnosis, Flow cytometry, Functional assay
نویسندگان مقاله | Mahshaad Norouzi
Blood Transfusion Research Center, High Institute for Education and Research in Transfusion Medicine, Tehran, Iran.


| Sahar Balagholi
Blood Transfusion Research Center, High Institute for Education and Research in Transfusion Medicine, Tehran, Iran.


| Amir Teimourpour
Blood Transfusion Research Center, High Institute for Education and Research in Transfusion Medicine, Tehran, Iran.


| Minoo Ahmadinejad
Blood Transfusion Research Center, High Institute for Education and Research in Transfusion Medicine, Tehran, Iran.
نشانی اینترنتی http://ijbc.ir/browse.php?a_code=A-10-1238-1&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Adults Hematology & Oncology
نوع مقاله منتشر شده پژوهشی
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