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Medical Laboratory Journal، جلد ۱۸، شماره ۴، صفحات ۱۷-۲۱
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عنوان انگلیسی Comparative evaluation of in vitro activity of tigecycline using the disc diffusion method and the VITEK-2 COMPACT in clinical isolates at a tertiary care cancer center
چکیده انگلیسی مقاله
Background: Tigecycline susceptibility testing and reporting remain enigmatic due to the lack of established guidelines. Disc diffusion, as a method of performing susceptibility testing, is more widely accepted worldwide due to its ease of use. Limited published literature is available from India on the utility of this method, especially in a cancer care setting. Hence, this study was conducted to evaluate the performance characteristics of disc diffusion by comparing its results with those of the VITEK-2 COMPACT, considering the latter as the standard.
Methods: Disc diffusion was performed using Kirby-Bauer’s method on Mueller-Hinton agar with a HiMedia 15 mcg TGC disc, following FDA and EUCAST breakpoints. According to CLSI criteria, disc diffusion breakpoints can be considered acceptable when categorical agreement is ≥ 90%, the very major error is ≤ 1.5%, and the major error is ≤ 3%.
Results: Using Cohen’s kappa coefficient, the kappa value was 0.328, with a p-value of <0.05. The agreement percentage observed was 60.84%. Two strains reported as resistant by VITEK-2 COMPACT were misclassified as sensitive by disc diffusion, resulting in a very major error rate of 0.76%. A major error rate of 9.5% and a minor error rate of 27.7% were noted, as 25 strains reported as susceptible were identified as resistant.
Conclusion: Since poor agreement was observed, exceeding the acceptable performance rate, the disc diffusion method was unacceptable according to CLSI criteria. There is a gap in uniformity and a lack of streamlined, harmonized TST, which might become an alarming cause for concern.
 
کلیدواژه‌های انگلیسی مقاله Tigecycline, Disk diffusion antimicrobial tests, Microbial drug resistance, Multiple drug resistance, Klebsiella pneumoniae, Broth microdilution test
نویسندگان مقاله | Sujata Lall
Department of Microbiology, HBNI ACTREC TMC, NAVI Mumbai, India


| Vivek Bhat
Department of Microbiology, HBNI ACTREC TMC, NAVI Mumbai, India


| Sanjay Biswas
Department of Microbiology, HBNITATA Memorial Hospital, Mumbai, India


| Navin Khattry
Department of Medical Oncology HBNI ACTREC, TMC, NAVI Mumbai, India
نشانی اینترنتی http://mlj.goums.ac.ir/browse.php?a_code=A-10-1645-1&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده میکروب شناسی
نوع مقاله منتشر شده Research Article
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