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Medical Laboratory Journal، جلد ۱۸، شماره ۴، صفحات ۲۴-۲۶
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عنوان انگلیسی Biofilm-forming ability and Agr-specific group of methicillin-resistant Staphylococcus aureus in Northern Iran
چکیده انگلیسی مقاله
Background: Staphylococcus aureus is one of the most common agents of nosocomial infection worldwide. Methicillin-resistant and biofilm-associated infections of this bacterium have become a clinical concern in patients. This research aimed to identify biofilm-forming ability and accessory gene regulator (Agr) - specific group of clinical isolates of methicillin-resistant S. aureus (MRSA) in Northern Iran.
Methods: In 2021, a total of 200 clinical isolates were identified as S. aureus by biochemical tests. The disk diffusion method was used to examine the antibiotic resistance of isolates and the microplate method was applied to investigate the biofilm production capability. In addition, the PCR method was used to determine the frequency of biofilm-associated genes and Agr typing of MRSA isolates. P £ 0.05 was considered significant.
Results: Overall, 62.5% of isolates were methicillin-resistant and 75% were multiple antibiotic-resistant. Biofilm-forming ability was detected in 99 (79.2%) methicillin-resistant isolates in which icaA and icaD were found in 85% and 78% of biofilm-producing isolates, respectively. Type 1 of the Agr gene was the most common type among methicillin-resistant isolates. The frequency of biofilm-associated genes showed a significant association with MDR phenotype and the presence of Agr locus (P £ 0.05).
Conclusion: The present findings indicate a high frequency of biofilm and antibacterial resistance in methicillin-resistant S. aureus isolates in Guilan Province. These findings suggest reliable and rapid identification of biofilm-forming MRSA strains to prevent the spread of these bacteria.
 
کلیدواژه‌های انگلیسی مقاله Agr protein, Biofilms, Staphylococcus aureus, Methicillin resistance
نویسندگان مقاله | Mahsa Aghaei
Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran


| Leila Asadpour
Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran


| Amir Arasteh
Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran
نشانی اینترنتی http://mlj.goums.ac.ir/browse.php?a_code=A-10-1216-2&slc_lang=en&sid=1
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زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده باکتری شناسی
نوع مقاله منتشر شده Research Article
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