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Cell Journal، جلد ۲۶، شماره ۱۱، صفحات ۶۲۵-۶۳۱
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عنوان انگلیسی Effect of Amyloid Beta on Cholesterol Metabolism-Correlated microRNAs in Primary Cultured Astrocytes of C57BL/6J Mice: A Focus on CYP46A1 and APOE Genes
چکیده انگلیسی مقاله Objective: The accumulation of amyloid plaques and disturbance of cholesterol homeostasis are implicated in the
pathophysiology of Alzheimer’s disease. Apolipoprotein E (ApoE) and cholesterol 24-hydroxylase (CYP46A1) are key
proteins involved in the efflux and metabolism of excess cholesterol, and small non-coding RNAs (miRNAs), can help
to regulate the expression of the genes encoding these proteins. The aim of the present study was to investigate
the alterations in the expression of APOE and CYP46A1 genes, as well as their respective regulatory miRNAs, in
astrocytes treated with amyloid beta (Aβ).
Materials and Methods: In this experimental study, isolated astrocyte cells were cultured and treated with Aβ for 24 hours. Changes in the expression of APOE and CYP46A1 genes, as well as their regulating miRNAs, were assessed using the realtime polymerase chain reaction (PCR) technique.
Results: The expression of APOE and CYP46A1 genes increased with Aβ treatment. MiR-33a-5p, as the negative
regulator of the APOE gene exhibited significant decrease. Additionally, miR-let-7a-5p, as the positive regulator of
the APOE gene, showed an increase in the Aβ treated group. Moreover, miR-98-5p, as the negative regulator of the
CYP46A1 gene, showed a half-fold decrease. While, miR-27a-3p as the positive regulator of the CYP46A1 gene,
increased significantly with Aβ treatment.
Conclusion: Alterations in the expression of APOE and CYP46A1 genes, as well as the expression of miRNAs
regulating these genes, in astrocytes treated with Aβ suggests that the cell is attempting to modify the regulatory
pathways of cholesterol homeostasis in the brain under pathological conditions, such as Alzheimer’s disease.
کلیدواژه‌های انگلیسی مقاله Amyloid beta-Peptides,Apolipoproteins E,Cholesterol,Cholesterol 24-Hydroxylase,MicroRNAs
نویسندگان مقاله Bahar Jaberian Asl |
Atherosclerosis Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Zahra Nazeri |
Department of Clinical Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Seyadeh Pardis Pezeshki |
Department of Clinical Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Alireza Kheirollah |
548-E Borwell Research Building, Geisel School of Medicine at Dartmouth, Hanover, NH 03755, USA

Shirin Azizidoost |
Atherosclerosis Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Maryam Adelipour |
Department of Clinical Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Maryam Cheraghzadeh |
Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

نشانی اینترنتی https://www.celljournal.org/article_720543_29ee4896f9e7ea1b53b3f7ed01780a0a.pdf
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