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Iranian Journal of Medical Sciences، جلد ۵۰، شماره ۴، صفحات ۲۴۷-۲۵۹
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عنوان انگلیسی Investigation of Lactobacillus Probiotics Derived from Traditional Dairy Products in Eliciting Anti-Tumor Responses in Mouse Colorectal Cancer Model
چکیده انگلیسی مقاله Background: Colorectal cancer (CRC) is a serious health problem, and finding new treatments is important. There is growing evidence for the antitumor activity of probiotics. This study investigated the anti-cancer potential of a probiotic mix containing Lactobacillus plantarum, L. brevis, L. helveticus, and L. delbrueckii, alone or in combination with the chemotherapy drug 5-fluorouracil, against CRC. 
Methods: The research was carried out in Mashhad in 2021. The cytotoxic effect of Lactobacillus isolates on CRC cells was investigated in two-dimensional and three-dimensional cell culture models. Histological staining and molecular approaches were used to investigate the regulatory mechanism of Lactobacillus isolates on cell migration, inflammation, fibrosis, cell cycle progression, apoptosis, and tumor necrosis in the CRC mouse model. Statistical analysis was performed using SPSS software version 20 with a significance level of P<0.05. The tests employed included the Kolmogorov–Smirnov, ANOVA, Dunnett’s post hoc, and Kruskal–Wallis.
Results: Lactobacillus strains effectively suppressed tumor growth in CRC by promoting cell death and inhibiting fibrosis and inflammation. These bacteria regulated apoptosis-related genes such as Bcl-2-associated protein x (P=0.0033), and BCL-2 (P=0.0029), leading to increased tumor necrosis. Treatment with bacterial supernatants reduced tumor size and fibrosis by downregulating collagen type I, alpha 1 (Col1a1) (P=0.024), Col1a2 (P=0.0231), and actin alpha 2 (P=0.0466), and transforming growth factor-beta expression. Additionally, they suppressed inflammation by decreasing tumor necrosis factor-alpha (P=0.0001), interleukin 6, and IL-1β (P=0.0198) levels in tumor tissues. Furthermore, the treatment inhibited CRC cell migration by modulating epithelial cadherin (P=0.0198) and matrix metallopeptidase 2 (P=0.033) expression.  
Conclusion: Findings indicated that co-administration of Lactobacillus isolates with 5-FU could improve the anti-tumor properties of the standard drug, 5-FU, supporting the therapeutic potential of these safe isolated lactic acid bacteria for CRC patients in vivo.
کلیدواژه‌های انگلیسی مقاله Probiotic,Lactobacillus,Colorectal cancer,Inflammation,fibrosis
نویسندگان مقاله Shaghayegh Rezai |
Department of Medical Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Elnaz Ghorbani |
Department of Medical Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Seyedeh Elnaz Nazari |
Department of Medical Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Farzad Rahmani |
Metabolic Syndrome Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

Seyed Mahdi Hassanian |
Metabolic Syndrome Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

Asma Afshari |
Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Mohammad Bagher Habibi Najafi |
Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

Amir Avan |
Metabolic Syndrome Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

Mikhail Ryzhikov |
Saint Louis University, School of Medicine, Saint Louis, MO, USA

Saman Soleimanpour |
Department of Medical Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Majid Khazei |
Department of Medical Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

نشانی اینترنتی https://ijms.sums.ac.ir/article_50612_e6517c56564c91c4b1868c7bc8098eee.pdf
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