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Medical Laboratory Journal، جلد ۱۸، شماره ۶، صفحات ۰-۰
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چکیده فارسی مقاله
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عنوان انگلیسی Molecular Characterization of Efflux Pumps in Beta-lactam Resistant Pseudomonas aeruginosa
چکیده انگلیسی مقاله Background: Pseudomonas aeruginosa is a common causative agent of hospital-acquired infections and exhibits resistance to many antibiotics, including beta-lactams. One of the mechanisms of resistance to beta-lactams is the MexAB-OprM efflux pump. This study investigates the genetic pattern of resistant P.aeruginosa strains concerning the presence of gene encoding the MexAB-OprM efflux pump.
Methods: One hundred and one clinical isolates of P.aeruginosa were collected from teaching hospitals in Isfahan between 2022 and 2023. Antibiotic susceptibility testing of the isolates was conducted using the disk diffusion method, and strains producing extended-spectrum beta-lactamases (ESBL) were identified by the combined disk method. The gene encoding the MexAB-OprM efflux pump in these strains was investigated by polymerase chain reaction (PCR).
Results: A significant proportion of the 101 P.aeruginosa isolates originated from the emergency department and ICU-2, highlighting the clinical significance of this pathogen in these settings. Meropenem demonstrated a high resistance rate (74%), while gentamicin exhibited lower resistance (33.33%). The prevalence of extended-spectrum beta-lactamases (ESBLs) was 30.3%, and the MexAB-OprM efflux pump gene was identified in 80% of ESBL-producing strains, suggesting a potential role in multidrug resistance.

 
کلیدواژه‌های انگلیسی مقاله MexAB-OprM efflux pump, Pseudomonas aeruginosa, extended-spectrum beta-lactamase
نویسندگان مقاله | Mozhgan Ghiasian
Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran


| Elmira Shah Cheraghi
Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
نشانی اینترنتی http://mlj.goums.ac.ir/browse.php?a_code=A-10-1803-1&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده میکروب شناسی
نوع مقاله منتشر شده Research Article
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