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Cell Journal، جلد ۲۲، شماره supplement ۱، صفحات ۱۰۱-۱۰۹
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عنوان انگلیسی Integrative Analysis Of LncRNAs In Kidney Cancer To Discover A New LncRNA (LINC00847) As A Therapeutic Target For Staphylococcal Enterotoxin Tst Gene
چکیده انگلیسی مقاله Objective: Bacterial toxin can cause cell death through induction of apoptosis in cancer cell lines as well as changes in the expression patterns of long non-coding RNAs (lncRNAs) and genes. In the present study, the effect of tst gene on ACHN cell lines was reported along with proposing a novel pathway of apoptosis in kidney cancer.
Materials and Methods: In this experimental study, effective lncRNAs and genes were predicted from different criteria
for renal cell carcinoma (RCC) by bioinformatics methods and lncRNA-miRNA-mRNA interaction was constructed; then the effect of Staphylococcus aureus tst gene on induction of apoptosis pathways on ACHN and HDF cell lines was investigated.
Results: After creation of lncRNA-miRNA-mRNA interaction, changes in expression levels of lncRNA LINC00847 (P=0.0024) and PTEN gene (P=0.0027) were identified, as potential apoptosis biomarkers for kidney cancer, after treating ACHN cell line by pCDNA3.1 (+)-tst compared to the empty vector. In contrast, there was no statistically significant difference in DICER1 expression levels in ACHN-tst cell (P≥0.05). In addition, transfection by pcDNA3.1 (+)-tst could increase ACHN cell apoptosis level (P<0.0001) compared to the pcDNA3.1 (+) group; but no significant effect was observed on normal cells.
Conclusion: It is suggested that lncRNA LINC00847, discovered in this study, could provide a new landscape for researches aimed to determine relationship between functional lncRNA and RCC pathways. pcDNA3.1 (+)-tst was
found to increase apoptosis in the transfected cells.
کلیدواژه‌های انگلیسی مقاله Apoptosis, Long Non-Coding RNA, MicroRNA, mRNA, TSST-1 Toxin
نویسندگان مقاله Maryam Safarpour-Dehkordi |
Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

Abbas Doosti |
Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

Mohammad-Saied Jami |
Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran

نشانی اینترنتی https://www.celljournal.org/article_251702_e06dd593b48d94caa95da65886ae074a.pdf
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