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Iranian Journal of Basic Medical Sciences، جلد ۲۸، شماره ۷، صفحات ۸۸۸-۸۹۸
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عنوان انگلیسی Daidzein improves neuronal health and alleviates inflammation and apoptosis through BDNF and estrogen receptors in the hippocampus of ovariectomized rats
چکیده انگلیسی مقاله Objective(s): Isoflavone Daidzein (DDZ) has emerged as a promising alternative to hormone replacement therapy (HRT) for ameliorating estrogen deficiency (ED). However, the stereological and molecular mechanism of its effects in the OVX-hippocampus are unclear. We studied the impact of DDZ on stereological changes, estrogen receptor (ERs) expression, BDNF, GSK-3β, and inflammatory and apoptosis-related genes in the hippocampus of ovariectomized rats, compared to 17β-estradiol (E2).Materials and Methods: OVX rats were treated with DDZ or E2. The stereological analysis assessed the total volume and number of pyramidal and granular neurons in the hippocampus CA1 and DG subregions. Expression of proinflammatory cytokines, apoptotic-related genes, ERs, and BDNF genes was evaluated using Real-Time PCR, and the GSK-3β phosphorylation level was measured by western blot analysis.Results: DDZ has effectively increased the volume and total number of pyramidal neurons in the CA1 region, the expression of ERα, ERβ, BDNF, and Bcl-2 genes, and the phosphorylation rate of GSK-3β protein. However, the effect of DDZ on the DG region, ERα, and BDNF genes was not significant in comparison with E2; DDZ significantly suppressed the expression of TNF-α, IL-6, and the Bax/Bcl2 ratio compared with OVX rats.Conclusion: DDZ effectively reversed the stereological changes in the CA1 region by stimulating BDNF gene expression, increasing the phosphorylation ratio of the GSK-3β protein, and modulating inflammatory and apoptotic pathways. Although its effects on the DG region, BDNF, and ERα molecules were less significant than E2, DDZ could still be a promising candidate for ameliorating ED.
کلیدواژه‌های انگلیسی مقاله CA1 region, Dentate gyrus, Estrogen deficiency Hippocampal, Menopause, Ovariectomy
نویسندگان مقاله | Asma Neisy
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran


| Zahra Khoshdel
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran


| Farhad Koohpeyma
Research committee, endocrine and metabolism research center, Shiraz University of Medical Sciences, Shiraz, Iran


| atefeh Seghatoleslam
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran


| Zohreh Mostafavi-Pour
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran


| Sanaz Alaee
Reproductive Biology Department, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran


| Fatemeh Keshavarzi
Autophagy Research Center, Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran


| Saeed Shokri
School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, Sydney, New South Wales, Australia


| Fatemeh Zal
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran|Infertility Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran
نشانی اینترنتی https://ijbms.mums.ac.ir/article_25793.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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