| چکیده انگلیسی مقاله |
Objective(s): Substrate engineering is one of the attractive fields of changing cell behavior and fate, especially for stem cell (SC) therapies. The SC pool is an essential factor in transplantation outcomes. Here, the objective was to preserve the stemness of the cornea’s limbal epithelial stem cell (LESC) using naturally biomimicking corneal cell topography.Materials and Methods: A cell-imprinted substrate was prepared using the natural topography of rabbit cornea’s LESC. The LESC cells were characterized by immunostaining (ABCG2 and Cytokeratin-12), then re-cultivated on a topography mold (imprinted PDMS), on FLAT PDMS (without any pattern), and the control group (tissue culture plate). Ultimately, an alkaline burn model was created on a rabbit’s cornea, and the effectiveness of cell-imprinted molds as implants for healing corneal wounds was examined in vivo. Results: The in vitro results showed that imprinted PDMS kept LESC cells in a state of stemness with high expression of ∆NP63 and ABCG2 genes (stemness-associated genes) compared to the other two groups and low Cytokeratin-3 and -12 expression (as differentiation-related genes). In vivo studies showed a more significant number of cells and the expression of the ABCG2 gene in the imprinted PDMS group. In contrast, higher expressions of the ∆Np63 gene and more stratification were observed in the control group (no treatment). Histological studies showed that the imprinted PDMS group had normal morphology with fully organized collagens. Conclusion: The results of LESC cultured on imprinted PDMS suggested that LESC cell imprinting could be an excellent substrate for LESC expansion and preserve their stemness for cell therapy. |
| نویسندگان مقاله |
| Tahereh Manoochehrabadi
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran|Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran
| Ali Samadikuchaksaraei
Department of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran
| Amin Solouki
Department of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
| Seyed-Hashem Daryabari
Basir Eye Health Research Center, Iran University of Medical Sciences, Tehran, Iran
| Hamed Ghasemi
Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran
| Ehsan Lotfi
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran|Department of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran
| Sajad Mansourian
Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran
| Jila Majidi
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran|Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran
| Peiman Brouki Milan
Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran
| Mazaher Gholipourmalekabadi
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran|Department of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran|Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran
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