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Iranian Journal of Medical Sciences، جلد ۵۰، شماره ۵، صفحات ۳۳۴-۳۵۰
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عنوان انگلیسی The Ameliorative Effect of Adipose-Derived Mesenchymal Stem Cells and Their Exosomes in Non-alcoholic Steatohepatitis by Simultaneously Enhancing Autophagic Flux and Suppressing Endoplasmic Reticulum Stress
چکیده انگلیسی مقاله Background: Due to the scarcity of treatment options, managing the progression of non-alcoholic fatty liver disease (NAFLD) from steatosis to cirrhosis necessitates innovative approaches. This study focused on endoplasmic reticulum (ER) stress, apoptosis, and autophagy as key mechanisms in NAFLD pathogenesis. It also highlighted the potential of adipose-derived mesenchymal stem cells (AD-MSCs) and their exosomes as promising therapeutic options.
Methods: The study was conducted at the Department of Regenerative Medicine, Shiraz University of Medical Sciences, (Shiraz, Iran) from November 2021 to December 2023. The mice (n=32) were divided into four groups: control, high-fat diet (HFD) without treatment, HFD with AD-MSCs treatment, and HFD with AD-MSCs-derived exosomes groups. The mice were fed HFD for 8 weeks. They received MSC and exosomes for the last 3 weeks. One week after the final injection, mice were tested for serum testing, stereological analysis, and real-time polymerase chain reaction (RT-PCR). The data were analyzed using the Graph-Pad Prism software by one-way analysis of variance (ANOVA) with Tukey analysis as a post hoc comparison between groups. P<0.05 indicated a significant difference. 
Results: AD-MSCs-exosomes significantly reduced ER stress indicators (IRE1α [P=0.0001], PERK [P=0.0006], ATF6 [P=0.0001], and GRP78 [P=0.0001]), apoptosis markers (Bax [P=0.005] and Cas3 [P=0.001]), and autophagic flux markers (P62 [P=0.0001] and LC3B/A [P=0.003]). 
Conclusion: In this investigation, AD-MSCs-exosomes significantly restored autophagy and suppressed unfolded protein response (UPR) pathways in the early stages of NAFLD.
کلیدواژه‌های انگلیسی مقاله Non-Alcoholic fatty liver disease,Mesenchymal Stem Cells,Exosome,Autophagy,endoplasmic reticulum stress
نویسندگان مقاله Zahra Moayedfard |
Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran

Kamran Bagheri Lankarani |
Health Policy Research Center (HPRC), Shiraz University of Medical Sciences, Shiraz, Iran.

Ali Akbar Alizadeh |
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran

Ali Akbar Nekooeian |
Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

Mahintaj Dara |
Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

Farhad Koohpeyma |
Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran

Shima Parsa |
Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

Saman Nikeghbalian |
Abu Ali Sina Hospital for Medicine and Organ Transplant, Shiraz University of Medical Sciences, Shiraz, Iran

Arghavan Hosseinpouri |
Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran

Negar Azarpira |
Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

نشانی اینترنتی https://ijms.sums.ac.ir/article_50757_7527c43e82dd45e57bdf82265506acff.pdf
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