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Iranian Journal of Medical Sciences، جلد ۴۲، شماره ۱، صفحات ۶۶-۰
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عنوان انگلیسی Beta-catenin Forms Protein Aggregation at High Concentrations in HEK293TCells
چکیده انگلیسی مقاله Background: The canonical Wnt signal transduction (or the Wnt/β-catenin pathway) plays a crucial role in the development of animals and in carcinogenesis. Beta-catenin is the central component of this signaling pathway. The activation of Wnt/β-catenin signaling results in the cytoplasmic and nuclear accumulation of β-catenin. In the nucleus, β-catenin interacts with the TCF/LEF transcription factors and, therefore, participates in the upregulation or downregulation of some important genes involved in diverse cellular activities. In addition, β-catenin is a critical component of the cadherin-mediated cell adherens junction. We had previously noticed that very high cellular concentrations of β-catenin had a negative effect on the transcriptional activity of this protein and, therefore, the aim of this study was to find a mechanism for this negative interaction. Methods: Cell fractionation, western blotting, and immunofluorescence microscopy experiments were performed to measure β-catenin protein levels and β-catenin cellular localization in HEK293Tcells transfected with various amounts of a β-catenin-encoding plasmid. Also, total RNA was extracted from the cells and used for reverse transcriptase-PCR experiments to measure the expression of the β-catenin target genes. SPSS, version 16, was used to analyze the results statistically. Results: We demonstrated that overexpression of β-catenin led to the formation of rod-shaped protein aggregates. The aggregate structures were mainly formed in the cell nucleus and were heavy enough to be isolated by centrifugation. Beta-catenin aggregate formation was accompanied by a decrease in the expression of theβ-catenin target genes used in this study. Conclusion: Since deregulation of β-catenin function occurs in several human diseases, including cancer and neurological disorders, the results of this paper further support the possible biological and clinical significance of β-catenin aggregate formation.
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نویسندگان مقاله ماریه سقاییان جزی | marie saghaeian jazi
department of molecular medicine, golestan university of medial sciences, gorgan, iran

سازمان اصلی تایید شده: دانشگاه گلستان (Golestan university)

سید محمود عرب نجفی | seyed mahmoud arab najafi
department of cell and molecular biology, school of biology, tehran university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

نشانی اینترنتی http://ijms.sums.ac.ir/index.php/IJMS/article/view/2226
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/147/article-147-350697.pdf
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article(s)
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