این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Journal of Arthropod-Borne Diseases، جلد ۱۸، شماره ۳، صفحات ۲۳۸-۲۵۲
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Purification and Molecular Characterization of a Mammalian Neurotoxin as a Pharmaceutical Tool from the Venom of Iranian Scorpion Androctonus crassicauda
چکیده انگلیسی مقاله Background: Venom of scorpions are complex bioactive polypeptides. To gain greater insights into the structural and functional impacts of toxins from Androctonus crassicauda (Buthidae), a dangerously venomous scorpion species, its venom was isolated, purified, and characterized.   Methods: Long chain toxin with four disulfide bonds purified by size exclusion chromatography and reversed-phase HPLC and characterized by amino acid sequencing and molecular weight determination. Results: The primary structure analysis exhibits a neurotoxin named AnCra2 with 7302.24 Da molecular weight and 64 amino acid residues that cause paralysis and lead to death in NIH mice. The LD50 of AnCra2 was determined to be 0.61±0.04 μg/mice. Phylogenetic analysis displays the toxin has 97% sequence similarity with alpha toxins reported from north African scorpions that affect voltage-gated sodium channels (VGSC), also proposed that differentiation among the scorpions of family Buthidae is affected by the geographical conditions and efficiency in evolutionary varia­tions. AnCra2 exposed binding residues have a high affinity for receptor residues in site-3 (segment-3) of VGSC that are approved by three-dimensional structure and homology modeling. Conclusion: Purified AnCra2 seems to be a new putative Alpha neurotoxin in homology with the structure of neurotox­ins that act on VGSC as a pharmaceutical tool.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | Hadi Rabiei
Department of Chemistry, Karaj Branch, Islamic Azad University, Karaj, Iran


| Abbas Zare Mirakabadi
Venomous Animals and Antivenom Production Department, Razi Vaccine and Serum Research Institute, Agricultural Research-Education and Extension Organization, Karaj, Iran


| Bita Mohtat
Department of Chemistry, Karaj Branch, Islamic Azad University, Karaj, Iran


| Behrooz Mirza
Department of Chemistry, Karaj Branch, Islamic Azad University, Karaj, Iran
نشانی اینترنتی https://jad.tums.ac.ir/index.php/jad/article/view/1582
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات